Genome editing of the SfABCC2 gene confers resistance to Cry1F toxin from Bacillus thuringiensis in Spodoptera frugiperda  

作　　者：JIN Ming-hui TAO Jia-hui LI Qp CHENG Ying SUN Xiao-xu WU Kong-ming XIAO Yu-tao 

机构地区：[1]Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen 518120,P.R.China [2]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,P.R.China

出　　处：《Journal of Integrative Agriculture》2021年第3期815-820,共6页农业科学学报（英文版）

基　　金：supported by the Key Project for Breeding Genetic Modified Organisms of China(2016ZX08012004003)。

摘　　要：ATP-binding cassette transporter C2(ABCC2)is known to be a receptor for Bacillus thuringiensis(Bt)toxins in several lepidopteran insects.Mutations in the ABCC2 gene have been genetically linked to field-evolved resistance to the Cry1 F toxin from Bt in Spodoptera frugiperda.Here we generated a SfABCC2 knockout strain of S.frugiperda using the CRISPR/Cas9 system to provide further functional evidence of the role of this gene in susceptibility and resistance to Cry1 F.Results from bioassays showed that the SfABCC2 knockout S.frugiperda strain displayed 118-fold resistance to Cry1 F compared with the parental DH19 strain,but no resistance to Vip3 A toxin from Bt.These results provide the first reverse genetic evidence for SfABCC2 as a functional receptor for Cry1 F.

关 键 词：Spodoptera frugiperda ABCC2 CRISPR/Cas9 Bt receptor Cry1F 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]