Susceptibility and tissue specificity of Spodoptera frugiperda to Junonia coenia densovirus  

作　　者：CHEN Zu-wen YANG Yan-chao ZHANG Jian-feng JIN Ming-hui XIAO Yu-tao XIA Zhi-chao LIU Yuan-yuan YU Sai-zhen YANG Yong-bo WANG Yuan LI Yi LIU Kai-yu 

机构地区：[1]School of Life Sciences,Central China Normal University,Wuhan 430079,P.R.China [2]Agricultural Genomics Institute,Chinese Academy of Agricultural Sciences,Shenzhen 518120,P.R.China [3]Medical College,Hubei University of Arts and Science,Xiangyang 441053,P.R.China [4]Hubei Engineering Research Center of Viral Vector,Wuhan University of Bioengineering,Wuhan 430415,P.R.China

出　　处：《Journal of Integrative Agriculture》2021年第3期840-849,共10页农业科学学报（英文版）

基　　金：supported by the National Key R&D Program of China(2017YFD0200400);the Natural Science Foundation of Hubei Province,China(2017CFB241)。

摘　　要：The fall armyworm,Spodoptera frugiperda,which destroys many economic crops such as rice and maize,has recently invaded China.Insect viruses as biological control agents play important roles in killing pests.One potential viral insecticide is the environmentally highly infective and virulent densovirus.We successfully rescued Junonia coenia densovirus(JcDV)using its infectious clone in different insect cell lines and larvae of three insect species.Results showed that the lysate of cultured insect cells transfected by the JcDV infectious clone killed the 2 nd instar S.frugiperda.The LD50 of homogenate from JcDV-infected Spodoptera litura to the 2 nd instar S.frugiperda(1.76×10^(8)viral genome copies per larva during 10 d post infection)was higher than that of the 2 nd instar S.litura(7.39×10^(7)Jc DV genome copies)or Helicoverpa armigera larvae(9.71×10^(7)JcDV genome copies).The LT50 of the S.litura homogenate(2.60×10^(9)viral genome copies each larva)to the 2 nd instar S.frugiperda was 6.96 d,longer than that of the S.litura(6.18 d)or the 2 nd instar H.armigera(5.94 d).JcDV could infect the fat body of H.armigera,but not S.frugiperda or S.litura.Although JcDV can infect all three lepidopteran species,their susceptibility to the virus differs.JcDV has great potential as a biological control agent against pests such as S.frugiperda.

关 键 词：Spodoptera frugiperda Junonia coenia densovirus rescue of virus SUSCEPTIBILITY biological control 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]