K-ras诱导细胞衰老与胃癌及癌前病变关系研究  

Relationship between K-ras-induced cellular senescence and gastric cancer and precancerous lesions

作　　者：王洁 张云清[1] 赵斌[1] 尹香利[1,2] WANG Jie;ZHANG Yunqing;ZHAO Bin;YIN Xiangli(Affiliated Hospital of Yan’an University,Yan’an 716000,China)

机构地区：[1]延安大学附属医院,陕西延安716000 [2]渭南市中心医院,陕西渭南714000

出　　处：《陕西医学杂志》2021年第3期370-373,共4页Shaanxi Medical Journal

基　　金：陕西省延安市科技攻关计划项目(2017KS-11-02)。

摘　　要：目的:对比胃癌与癌前病变组织中K-ras蛋白表达的差异,分析K-ras诱导的细胞衰老与胃癌及癌前病变的关系。方法:收集138例管状腺癌患者的病理样本,进行HE染色和免疫组化染色,检测胃癌及癌旁石蜡包埋组织中K-ras、SA-β-Gal、P53、P16~(INK4a)蛋白的表达。结果:K-ras蛋白在胃癌组织中阳性表达率显著高于癌旁组织(95.7%与3.6%)(P<0.05)。胃癌组织及癌旁组织中K-ras阳性组SA-β-Gal、P53、P16~(INK4a)表达率均高于K-ras阴性组(均P<0.05)。结论:K-ras诱导的细胞衰老与胃癌的发生、发展密切相关。Objective:To compare the expression of K-ras protein in gastric cancer and precancerous lesions,and to analyze the relationship between K-ras-induced cellular senescence and gastric cancer and precancerous lesions.Methods:138 gastric tubular adenocarcinoma specimens were collected,and HE staining and immunohistochemical staining were performed.The expression of K-ras,SA-β-Gal,P53 and P16 INK4a in gastric cancer and adjacent tissues were detected.Results:The positive expression rate of K-ras protein in gastric cancer tissues was significantly higher than that in adjacent tissues(95.7%vs.3.6%)(P<0.05).The positive rates of SA-β-Gal,P53 and P16 INK4a expression of K-ras positive group in gastric cancer tissues and adjacent tissues were higher than those in K-ras negative group(all P<0.05).Conclusion:K-ras-induced cellular senescence is closely related to the occurrence and development of gastric cancer.

关 键 词：胃癌 癌前病变 K-RAS 细胞衰老 免疫组化 

分 类 号：R735.2[医药卫生—肿瘤]