运用高频超声对肝硬化所致大鼠心肌损害及颈部血管病变的评估价值研究  

Study on the evaluation of myocardial damage and cervical vascular disease caused by liver cirrhosis using high-frequency ultrasound

作　　者：徐晓申 郭萌[1] 杨青[1] XU Xiaoshen;GUO Meng;YANG Qing(Department of Ultrasound Diagnosis,the Fifth Affiliated Hospital of Zhengzhou University,Zhengzhou 450000,China)

机构地区：[1]郑州大学第五附属医院超声诊断科,郑州450000

出　　处：《中国比较医学杂志》2021年第2期67-73,97,共8页Chinese Journal of Comparative Medicine

基　　金：河南省医学科技攻关计划(201503132)。

摘　　要：目的评估高频超声对肝硬化所致大鼠心肌损害及颈部血管病变的准确性。方法大鼠分为模型组和对照组,9周后,检测2组心功能及颈总动脉相关指标;HE染色观察肝、心脏及颈总动脉病理学变化;采用Pearson相关系数法分析超声测量左心室重量(LVM)与实测LVM及超声颈总动脉管壁厚度与HE染色颈总动脉内膜厚度测量值的相关性。结果对照组肝、心肌组织及颈总动脉结构均正常,模型组出现假小叶,肝细胞排列紊乱,中央静脉消失或偏向边缘,出现细胞变性及炎性细胞浸润,心肌细胞结构松散、肿胀,肌纤维走向混乱,出现断裂及炎性细胞浸润,细胞外间质增多,颈总动脉血管内壁增厚,管腔变窄,管腔面积变小。模型组HE染色颈总动脉内膜的测量值、实测LVM大于对照组(P<0.05);模型组心脏左心室舒张期末内径(LVEDD)、左心室收缩期末内径(LVESD)、左心室舒张末期容积(LVEDV)、LVM、心率(HR)高于对照组,左心室轴缩短率(FS)低于对照组(P<0.05);模型组颈总动脉血管内径、管壁厚度、血流速度小于对照组,脉动指数(PI)、阻力指数(RI)高于对照组(P<0.05);经Pearson相关性分析,超声LVM与实测LVM呈正相关(r=0.875,P<0.001);超声颈总动脉管壁厚度与HE染色颈总动脉内膜厚度的测量值呈正相关(r=0.821,P<0.001)。结论运用高频超声对肝硬化大鼠心肌损害及颈部血管病变进行评估,结果准确可靠,值得临床推广。Objective To evaluate the accuracy of high-frequency ultrasound in detecting myocardial damage and cervical vascular lesions caused by liver cirrhosis in rats.Methods Rats were divided into a model group and a control group.After 9 weeks,the cardiac function and common carotid artery-related indicators were detected in the two groups,and HE staining was used to observe the pathological morphological changes of the liver,heart,and common carotid artery.Pearson’s correlation coefficient was used to analyze the correlation between the actual left ventricular weight(LVM),ultrasound LVM,common carotid artery wall thickness determined by ultrasound,and actual thickness of HE staining common carotid intima.Results The structures of the liver,myocardial tissue,and common carotid artery in the control group were normal.In contrast,we observed false leaflets,disorganized liver cells,absent or edged central veins,various degrees of liver cell degeneration and inflammatory cell infiltration,and a loose and swollen structure of myocardial cells in the model group.In addition,myocardial fibers were disorganized,broken,and infiltrated by inflammatory cells,the extracellular matrix was increased,the inner wall of the common carotid artery was thicker,and the lumen was narrowed and reduced in size.The actual LVM and measured of HE staining common carotid artery intima in the model group were greater than those in the control group(P<0.05).The left ventricular end-diastolic diameter,left ventricular end-systolic diameter,left ventricular end-diastolic volume,LVM,and heart rate in the model group were higher than that in the control group,and fraction shortening was lower in the model group than in the control group(P<0.05).The carotid artery inner diameter,tube wall thickness,and blood flow velocity were reduced compared with the control group,and the pulsation index and resistance index were higher in the model group than in the control group(P<0.05).Pearson’s correlation analysis showed that the ultrasound LVM was positively

关 键 词：肝硬化 高频超声 心肌 颈总动脉 大鼠 

分 类 号：R-33[医药卫生]