一种SD大鼠高血脂模型建立方法的验证以及造模时长对相关指标的影响探讨  

Establishment of a hyperlipidemia Sprague-Dawley rat model and investigation of relevant indicators

作　　者：蔡拓 张慈 易吉平 余晓巍 CAI Tuo;ZHANG Ci;YI Jiping;YU Xiaowei(Hunan Prevention and Treatment Institute for Occupational Disease,Changsha 410007,China;Xiangya School of Nursing,Central South University,Changsha 410013)

机构地区：[1]湖南省职业病防治院,长沙410007 [2]中南大学湘雅护理学院,长沙410013

出　　处：《中国比较医学杂志》2021年第2期74-79,共6页Chinese Journal of Comparative Medicine

基　　金：湖南省自然科学基金(2019JJ50341)。

摘　　要：目的通过喂养SD大鼠高脂饲料诱导高血脂动物模型,比较造模8 d和14 d的差异,探讨模型中相关检测指标的变化规律,为降血脂评价体系中动物模型的建立提供参考依据。方法依照同一标准在相同环境条件下进行8 d和14 d两次造模,每次造模将72只雄性SD大鼠随机分为模型组和对照组,用喂饲法分别给予模型饲料和维持饲料,造模结束后测定动物各项指标。结果两次造模模型组动物血清中总胆固醇、甘油三酯、低密度脂蛋白-胆固醇均高于对照组(P<0.01),高密度脂蛋白-胆固醇均低于对照组(P<0.01);模型组之间及对照组之间各项指标相关性趋势高度一致,但模型组与对照组比较,检测指标的相关性均存在较大差异;模型组之间动物血清中甘油三酯、高密度脂蛋白含量均有明显差异(P<0.01);造模8 d、14 d动物均出现不同程度的肝病理性改变。结论两次造模均成功,且同为混合型高脂血症动物模型;使用该方法建立的高血脂模型具有造模时间短、关键指标稳定及重现性好的特点。Objective To induce a hyperlipidemic animal model by feeding Sprague-Dawley(SD)rats a high-fat diet,explore the variation in relevant indicators by comparing the difference between 8 and 14 days of modeling,and provide a reference for the establishment of animal models to facilitate the evaluation of hypolipidemic agents.Methods Models were established in 8 and 14 days under the same environmental conditions according to the same standards.Seventy-two male SD rats were randomly divided into the model and control groups.Model feed was given to the model groups,and maintenance feed was given to the control groups.The indicators of hyperlipidemia were evaluated in the established SD models.Results The total cholesterol,triglyceride,and low-density lipoprotein cholesterol levels in the model group were significantly higher than those in the control group(P<0.01).High-density lipoprotein cholesterol was lower than that in the control group(P<0.01).The correlation trend of each index between the two model groups was highly consistent.Similarly,the correlation trend of each index between the two control groups was highly consistent.However,the correlation between the model group and the control group was quite different.The serum triglyceride and high-density lipoprotein levels in the two model groups were significantly different(P<0.01).Liver pathological changes of different degrees were observed 8 and 14 days after modeling.Conclusions Hyperlipidemia models were successfully induced in both 8 and 14 days.They were both mixed hyperlipidemia models.The method used in this study is feasible,stable,and reproducible.

关 键 词：高脂血症 SD大鼠 动物模型 

分 类 号：R-33[医药卫生]