淡紫灰链霉菌X33水溶性抗真菌产物高产菌株的诱变选育  

Mutation screening for high-yield strains of a water-soluble fungicide from Streptomyces lavendulae X33

作　　者：杨晨军 汪林兰 余健 吴晓玉[1] Yang Chen-jun;Wang Lin-lan;Yu Jian;Wu Xiao-yu(College of Bioscience and Bioengineering,Jiangxi Agriculture University/Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources,Nanchang 330045)

机构地区：[1]江西农业大学生物科学与工程学院/江西农业微生物资源开发与利用工程实验室,南昌330045

出　　处：《中国抗生素杂志》2021年第2期121-127,共7页Chinese Journal of Antibiotics

基　　金：江西省重点项目“柑橘采后贮藏的微生物源生物保鲜剂的研究与开发(No.20171ACF60006)”;江西省2011果蔬采后处理关键技术及质量安全协同创新中心资助。

摘　　要：以淡紫灰链霉菌X33(Streptomyces lavendulae X33)野生菌为出发菌株,柑橘致病菌指状青霉(Penicillium digitatum)为指示菌,诱变选育对柑橘采后致病青霉具有显著抑制作用的水溶性抗真菌产物的高产菌株。经自然分离选育后,采用紫外线、亚硝基胍、五溴尿嘧啶与二氨基嘌呤混合三轮系列诱变处理,筛选获得一株性能稳定的高产诱变菌株BP39,其相对抑菌效价较出发菌株X33提高了279%。表明传统的物理、化学诱变选育方法,可大幅提高野生菌株活性产物产量,是一种简便、快速的育种手段。The high-yield strains produced a water-soluble fungicide for the control of postharvest pathogenic Penicillium of citrus was selected using the wild strain of Streptomyces lavendulae X33 as the starting strain and Penicillium digitatum as the indicator. A high-yield mutant strain S. lavendulae BP39 with genetic stability was obtained by three rounds of mutagenic treatment of ultraviolet, nitrosoguanidine, 5-bromouracil, and diaminopurine after natural screening. Its relative potency increased by 279% compared with the starting strain S. lavendulae X33. The results indicated that the methods employed by traditional physical and chemical mutagenesis could greatly improve the yield of secondary metabolites by wild strains, and it was a simple and rapid breeding way.

关 键 词：淡紫灰链霉菌X33 诱变育种 抗真菌产物 柑橘青霉 

分 类 号：R978.5[医药卫生—药品]