维生素C对牙周膜干细胞中HDAC1和HDAC6表达的影响  

Vitamin C affects the expression of HDAC1 and HDAC6 in periodontal ligament stem cells

作　　者：刘瑞琪 刘凡[1] 贾智[1] LIU Rui-qi;LIU Fan;JIAZhi(Department of Endodontics,Stomatology Hospital,Tianjin Medical University,Tianjin 300070,China)

机构地区：[1]天津医科大学口腔医院综合科,天津300070

出　　处：《天津医科大学学报》2021年第2期105-107,146,共4页Journal of Tianjin Medical University

摘　　要：目的:探究维生素C对牙周膜干细胞组蛋白去乙酰化酶(HDAC)1和HDAC6表达的影响。方法:收取临床上因正畸需要拔除的无龋坏前磨牙,分离并培养人牙周膜干细胞。流式细胞仪测定牙周膜干细胞表面标志物。qPCR检测添加5、20、80、320 mmol/L维生素C后,人牙周膜干细胞中HDAC1和HDAC6 mRNA的表达情况。结果:维生素C促进HDAC1(F=45.76,P<0.01)、HDAC6(F=119,P<0.01)的表达,且随着浓度的增加,HDAC1、HDAC6表达显著增加。结论:维生素C促进牙周膜干细胞中HDAC1和HDAC6表达。Objective:To reveal the effects of vitamin C on the expression of HDAC1 and HDAC6 mRNA in periodontal ligament stem cells.Methods:Human periodontal ligament stem cells were isolated and cultured from caries free premolars.Determination of surface markers of periodontal ligament stem cells by flow cytometry.qPCR was used to analyze the expression of HDAC1 and HDAC6 mRNA in human periodontal ligament stem cells treated with different concentrations of vitamin C(5,20,80,320 mmol/L).Results:Vitamin C promoted the expression of HDAC1 and HDAC6,and the expression of HDAC1(F=45.76,P<0.01)and HDAC6(F=119,P<0.01)increased significantly with the increase of vitamin C concentration.Conclusion:The results of the experiment indicate that vitamin C promotes the expression of HDAC1 and HDAC6 in periodontal ligament stem cells.

关 键 词：组蛋白去乙酰化酶 人牙周膜干细胞 维生素C HDAC1 HDAC6 

分 类 号：R781.4[医药卫生—口腔医学]