CXCL-14在非小细胞肺癌中的表达水平及临床意义  

The expression level and clinical significance of CXCL-14 in non-small cell lung cancer

作　　者：王红军[1] 贾金广 朱婉凌[1] 魏然[1] 姚菲菲[1] WANG Hong-jun;JIA Jin-guang;ZHU Wan-ling;WEI Ran;YAO Fei-fei(Department of Respiratory and Critical Care Medicine,People′s Hospital of Zhengzhou,Zhengzhou 450000,China)

机构地区：[1]郑州人民医院呼吸与危重症医学科,郑州450000

出　　处：《天津医科大学学报》2021年第2期112-116,共5页Journal of Tianjin Medical University

摘　　要：目的:检测CXC趋化因子配体14(CXCL-14)在非小细胞肺癌(NSCLC)中的表达水平并分析其临床意义。方法:收集2017年1月—2019年1月在郑州人民医院接受手术治疗的NSCLC患者260例。检测NSCLC组织、癌旁组织、NSCLC细胞A549、H1975、PC-9和正常肺上皮细胞BEAS-2B细胞CXCL-14 mRNA的相对表达水平;将siCXCL-14^(1)(siCXCL-14^(1)组)、siCXCL-142(siCXCL-142组)和si-RNA(对照组)质粒转染至A549细胞中,检测转染后细胞的增殖和凋亡情况;根据CXCL-14的平均表达水平将患者分为CXCL-14高表达组(n=138)与CXCL-14低表达组(n=122),分析各组患者的临床特征。结果:CXCL-14 mRNA在NSCLC组织中的相对表达水平为(3.62±0.78),高于癌旁组织(1.17±0.32),差异有统计学意义(t=22.440,P<0.001)。CXCL-14 mRNA在A549、H1975和PC-9中的相对表达水平分别为(2.10±0.13)、(1.84±0.21)和(1.47±0.08),均高于正常肺上皮细胞(0.69±0.08),差异有统计学意义(q=23.631、17.882、13.568,P<0.001);siCXCL-14^(1)组和siCXCL-142组CXCL-14 mRNA和蛋白相对表达水平均低于对照组,差异有统计学意义(均P<0.05);72 h时siCXCL-14^(1)组和siCXCL-142组细胞的OD值分别为(1.02±0.19)和(0.98±0.12),均低于对照组(1.63±0.21),差异有统计学意义(q=3.102,P=0.015;q=3.241,P=0.012);siCXCL-14^(1)组和siCXCL-142组细胞凋亡率分别为(13.57±2.82)%和(14.41±2.14)%,高于对照组的(5.58±0.69)%,差异有统计学意义(q=4.774,P=0.009;q=7.031,P=0.002);CXCL-14高表达组低分化、病理分期Ⅱ、Ⅲ期和淋巴结转移的患者多于CXCL-14低表达组,1年无进展生存的患者少于CXCL-14低表达组,差异均有统计学意义(均P<0.05)。结论:CXCL-14在NSCLC中表达增加,降低CXCL-14的表达可减弱细胞的增殖、促进细胞凋亡,CXCL-14表达水平增加可反映患者的不良预后。Objective:To detect the expression level of CXC chemokine ligand 14(CXCL-14)in non-small cell lung cancer(NSCLC)and analyze its clinical significance.Methods:A total of 260 patients with NSCLC who underwent surgical treatment at People′s Hospital of Zhengzhou from January 2017 to January 2019 were collected.The expression of CXCL-14 mRNA in NSCLC tissues,paracancerous tissues,NSCLC cells A549,h1975,PC-9 and BEAS-2B cells were detected;siCXCL-14^(1)(siCXCL-14^(1) group),siCXCL-142(siCXCL-142 group)and si-RNA(control group)plasmids were transfected into A549 cells.The proliferation and apoptosis of transfected cells were detected.According to the average level of CXCL-14 expression,patients were divided into CXCL-14 high expression group and CXCL-14 low expression group,and the clinical characteristics of patients between different groups were analyzed.Results:The relative expression level of CXCL-14 mRNA in NSCLC tissues was(3.62±0.78),higher than that of adjacent tissues[(1.17±0.32)],the difference was statistically significant(t=22.440,P<0.001).The relative expression levels of CXCL-14 mRNA in A549,H1975 and PC-9 were(2.10±0.13),(1.84±0.21)and(1.47±0.08),respectively,which were higher than that in normal lung epithelial cells(0.69±0.08),and the difference was statistically significant(q=23.631,17.882,13.568,P<0.001).The relative expression levels of CXCL-14 mRNA and protein in siCXCL-14^(1) group and siCXCL-142 group were lower than those in control group,and the difference was statistically significant(P<0.05).At 72 h,the OD values of cells in the siCXCL-14^(1) group and siCXCL-142 group were(1.02±0.19)and(0.98±0.12),both of which were lower than those in control group(1.63±0.21),the difference was statistically significant(q=3.102,P=0.015;q=3.241,P=0.012);the apoptosis rates of siCXCL-14^(1) group and si CXCL-142 group were(13.57±2.82)%and(14.41±2.14)%,which were higher than that of control group(5.58±0.69)%,the difference was statistically significant(q=4.774,P=0.009;q=7.031,P=0.002);there were

关 键 词：非小细胞肺癌 CXC趋化因子配体14 治疗靶点 

分 类 号：R734.2[医药卫生—肿瘤]