超高效液相色谱-串联质谱法测定鸡胗和鸡肝中氯霉素残留  

Residue determination of chloramphenicol residues in chicken gizzard and liver by ultra ultra-high performance liquid chromatography-tandem mass spectrometry

作　　者：庹见伟 刘君[1] 孙路明 王小琴 荣晓夙 TUO Jian-wei;LIU Jun;SUN Lu-ming;WANG Xiao-qin;RONG Xiao-su(Akesu Regional Center for Disease Control and Prevention,Akesu,Xinjiang 843000,China)

机构地区：[1]阿克苏地区疾病预防控制中心,新疆阿克苏843000

出　　处：《中国卫生检验杂志》2021年第3期287-289,293,共4页Chinese Journal of Health Laboratory Technology

摘　　要：目的建立测定鸡胗、鸡肝中氯霉素残留的超高效液相色谱-串联质谱(UPLC-MS/MS)分析方法。方法样品经乙酸乙酯提取,正己烷净化后,用UPLC-MS/MS在多反应监测(MRM)模式下进行检测,同位素内标法定量。结果氯霉素在0.10 ng/ml-10.00 ng/ml呈现良好的线性关系,相关系数>0.997,检出限为0.01μg/kg,当加标水平为0.4μg/kg,1.0μg/kg、10μg/kg时,氯霉素在鸡胗和鸡肝中的加标回收率为80.3%-92.9%,相对标准偏差(RSD)为1.6%~4.5%。结论该方法具有样品前处理简单快速、检测灵敏度高、方法重现性好等优点,可满足鸡胗、鸡肝中氯霉素残留的快速筛查和确证分析的要求。Objective An ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was established for the determination of chloramphenicol residues in chicken gizzards and livers.Methods The sample was extracted by ethyl acetate and then purified by n-hexane,the analytes were detected by UPLC-MS/MS in multi-reaction monitoring(MRM)mode and quantified by isotope internal standard method.Results Chloromycetin showed good linearity in the range of 0.10 ng/ml-10.00 ng/ml with good correlation coefficient(r)greater than 0.997 and the detection limit was 0.01 g/kg.The recoveries was within 80.3%-92.9%in chicken gizzards and livers when the spiked levels were 0.4μg/kg,1.0μg/kg,10μg/kg and the relative standard deviation(RSD)was within 1.6%-4.5%.Conclusion This method is simple and rapid in sample pretreatment,and has high detection sensitivity and good reproducibility.Therefore,it is suitable for the rapid screening and analysis of chloramphenicol residues in chicken gizzards and livers.

关 键 词：鸡胗 鸡肝 氯霉素 超高效液相色谱-串联质谱 

分 类 号：O657.63[理学—分析化学]