苦参素对肿瘤源性内皮细胞生物学行为的作用及其分子机制  

Effect of oxymatrine on biological behavior of tumor-derived endothelial cells and its molecular mechanism

作　　者：覃小珊[1,2] 张彩灵 黄赞松 Qin Xiaoshan;Zhang Cailing;Huang Zansong(Department of Gastroenterology,The Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;Graduate School,Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;Guangxi Clinical Medical Research Center for Hepatobiliary Diseases,Baise 533000,Guangxi,China)

机构地区：[1]右江民族医学院附属医院消化内科,广西百色533000 [2]右江民族医学院研究生学院,广西百色533000 [3]广西肝胆疾病临床医学研究中心,广西百色533000

出　　处：《右江民族医学院学报》2021年第1期17-21,共5页Journal of Youjiang Medical University for Nationalities

基　　金：广西自然科学基金项目(2014GXNSFAA118143);广西科技基地和人才专项(广西肝胆疾病临床医学研究中心研究课题)(桂科AD17129025);广西医药卫生计划课题(Z20170224);广西研究生教育创新计划项目(YCSW2020235)。

摘　　要：目的研究苦参素对肿瘤源性内皮细胞(Td-EC)增殖、迁移及成管能力的作用及其可能的分子机制。方法采用人肝癌HepG2细胞条件培养基将人脐静脉内皮细胞(HUVEC)诱导形成Td-EC,CCK-8法检测浓度分别为0.375 mg/ml、0.75 mg/ml、1.5 mg/ml、3 mg/ml、6 mg/ml的苦参素作用Td-EC 24 h的细胞增殖抑制率,选择IC 50浓度(3.5 mg/ml)的苦参素作用Td-EC 24 h,细胞划痕实验检测Td-EC的迁移能力及体外血管形成实验检测Td-EC的管形成能力,qRT-PCR检测Ets-1 mRNA的表达变化。结果CCK-8结果显示,0.375 mg/ml、0.75 mg/ml、1.5 mg/ml、3 mg/ml、6 mg/ml苦参素作用Td-EC 24 h后,各组细胞增殖均受到抑制。经3.5 mg/ml苦参素作用后,Td-EC的迁移能力及管形成能力降低,Td-EC中的Ets-1 mRNA表达降低,与阴性对照组相比,差异均有统计学意义(P<0.05)。结论苦参素可抑制Td-EC的增殖、迁移及成管能力,其分子机制可能与抑制Ets-1 mRNA的表达有关。Objective To explore the effect of oxymatrine on the proliferation,migration and tube-forming ability of tumor-derived endothelial cells(Td-EC)and possible molecular mechanism.Methods Human umbilical vein endothelial cells(HUVEC)were induced to form Td-EC in the conditioned medium of human liver cancer HepG2 cells.CCK-8 assay was used to detect proliferation inhibition rate of Td-EC treated for 24 h with oxymatrine at concentrations of 0.375 mg/ml,0.75 mg/ml,1.5 mg/ml,3 mg/ml and 6 mg/ml,respectively.The oxymatrine with 50%inhibitory concentration(3.5 mg/ml)was selected to treat Td-EC for 24 h.The migration ability of Td-EC was detected by cell scratch test and the tube-forming ability by in vitro angiogenesis test.The change of Ets-1 mRNA expression was detected by qRT-PCR.Results The CCK-8 assay showed that the proliferation of Td-EC was inhibited after treatment for 24 h with oxymatrine at concentrations of 0.375 mg/ml,0.75 mg/ml,1.5 mg/ml,3 mg/ml,6 mg/ml.After treated with 3.5 mg/ml oxymatrine,the migration ability and tube-forming ability of Td-EC decreased,and the expression of Ets-1 mRNA in Td-EC decreased,compared with the negative control group,the differences were statistically significant(P<0.05).Conclusion Oxymatrine can inhibit the proliferation,migration and tube-forming ability of Td-EC,and the molecular mechanism may be related to the inhibition of Ets-1 mRNA expression.

关 键 词：苦参素 原发性肝肿瘤 肿瘤源性内皮细胞 血管生成 E26转化特异性序列1 

分 类 号：R735.7[医药卫生—肿瘤]