PDGFA 在鼻咽癌中的差异表达及其功能分析  

Differential expression and functional analysis of PDGFA in nasopharyngeal carcinoma

作　　者：唐玉莲[1] 陈启威[2] 韦贵将[2] 李玉颖 候沅林 卢诗丽 刘洁兰 王太重[1] Tang Yulian;Chen Qiwei;Wei Guijiang;Li Yuying;Hou Yuanlin;Lu Shili;Liu Jielan;Wang Taizhong(School of Medical Laboratory,Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China;The Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,Guangxi,China)

机构地区：[1]右江民族医学院医学检验学院,广西百色533000 [2]右江民族医学院附属医院,广西百色533000

出　　处：《右江民族医学院学报》2021年第1期46-50,共5页Journal of Youjiang Medical University for Nationalities

基　　金：2018年右江民族医学院校级科研课题(yy2018ky015)。

摘　　要：目的探讨血小板衍生生长因子A(platelet-derived growth factor subunit A,PDGFA)在鼻咽癌中的差异表达和可能的功能作用。方法利用基因芯片数据库(GEO数据库)中的基因表达谱数据,通过GEO2R在线分析工具分析PDGFA基因在正常鼻咽黏膜组织与鼻咽癌组织中的差异表达情况,并通过STRING数据库和DAVID数据库分析该基因及其相关基因的功能作用,最后通过GCBI在线实验室分析其可能参与的共表达调控网络,并预测其转录调控因子。结果发现PDGFA基因在正常鼻咽黏膜组织与鼻咽癌组织中具有差异表达。PDGFA基因及其相关基因功能富集分析发现,它们共同参与了12个生物学过程、4个细胞组分、3个分子功能和11个信号通路,并在肿瘤的发生、发展和转移过程中均具有显著的富集作用,如MAPK信号通路、Jak-STAT信号通路、细胞增殖的正调控、细胞迁移的正调控、黏着、ERK1和ERK2级联的正调节、蛋白质自磷酸化的正调控等。另基因共表达调控网络和转录因子预测发现,PDGFA基因与许多基因、蛋白、转录因子以及microRNA具有相互作用。结论PDGFA可能通过多种途径,如细胞增殖和迁移的正调控、信号转导、蛋白质自磷酸化以及分子间的相互作用等参与鼻咽癌的发生、发展和迁移。Objective To investigate the differential expression of platelet-derived growth factor subunit A(PDGFA)in nasopharyngeal carcinoma(NPC)and its possible functions.Methods With the gene expression profile data in the GEO Database,we analyzed the differential expression of PDGFA gene in normal nasopharyngeal mucosa tissues and NPC tissues by online analysis tool GEO2R.Then the function and effect of PDGFA gene and its related genes were analyzed through STRING database and DAVID database.Finally,on GCBI online laboratory,we analyzed the co-expression regulatory network in which it may participate as well as predicted its transcription regulatory factors.Results PDGFA was differentially expressed in normal nasopharyngeal mucosa tissues and NPC tissues.Functional enrichment analysis of PDGFA and related genes showed that they jointly participated in 12 biological processes,4 cellular components,3 molecular functions and 11 signaling pathways,and they had significant enrichment effects in the process of tumor occurrence,development and metastasis.What they participated in included MAPK signaling pathway,Jak-STAT signaling pathway,positive regulation of cell proliferation,positive regulation of cell migration,adhesion,positive regulation of ERK1 and ERK2 cascade,and positive regulation of protein autophosphorylation,etc.In addition,gene co-expression regulatory network and transcription factor prediction revealed that PDGFA gene interacted with many genes,proteins,transcription factors and microRNA.Conclusion PDGFA may be involved in the occurrence,development and migration of NPC through multiple pathways,such as positive regulation of cell proliferation and migration,signal transduction,protein autophosphorylation,and intermolecular interactions.

关 键 词：PDGFA 鼻咽肿瘤 差异表达 功能分析 基因芯片 调控网络 

分 类 号：R739.03[医药卫生—肿瘤]