基于双十二烷基二甲基溴化铵、琼脂糖修饰电极的中心蛋白金属结合性质研究  

The studies on the metal binding characteristic of centrin based on Didodecyldimethylammonium bromide and agarose modified electrode

作　　者：荣志江 孙红艳[1] 杨艳[1] 周焕新 杨斌盛[2] Rong Zhijiang;Sun Hongyan;Yang Yan;Zhou Huanxin;Yang Binsheng(School of Chemical and Biological Engineering,Taiyuan University of Science and Technology,Taiyuan 030024,China;Institute of Molecular Science,Shanxi University,Taiyuan 030006,China)

机构地区：[1]太原科技大学化学与生物工程学院,太原030024 [2]山西大学分子科学研究所,太原030006

出　　处：《河南师范大学学报（自然科学版）》2021年第1期50-56,共7页Journal of Henan Normal University(Natural Science Edition)

基　　金：国家自然科学基金(21571117,31401319);山西省重点研发计划项目(201903D221066);山西省回国留学人员科研资助项目(2017-083);太原科技大学博士科研基金(20162018).

摘　　要：应用热解石墨电极(pyrolytic graphite electrode,PG)、双十二烷基二甲基溴化铵(Didodecyldimethylammonium bromide,DDAB)修饰电极,以及应用琼脂糖修饰电极研究了八肋游仆虫N端中心蛋白(N-terminal ciliate Euplotes octocarinatus centrin,N-EoCen)、C端中心蛋白(C-EoCen)与Eu^(3+)结合反应的循环伏安特性.研究发现在琼脂糖修饰电极上出现了配合物Eu^(3+)-N-EoCen一对氧化还原峰,与仅有Eu^(3+)存在时相比,负向移动0.24 V.在DDAB修饰电极上出现了配合物Eu^(3+)-N-EoCen微弱的不可逆还原峰.经过计算N-EoCen,C-EoCen与Eu^(3+)结合反应的结合常数β和结合数n,显示N-EoCen与Eu^(3+)形成1∶2型分子复合物,结合常数β=1.26×10^(7) L·mol^(-1);C-EoCen与Eu^(3+)结合比为1∶2,结合常数β=1.38×10^(9) L·mol^(-1).The interaction between N-terminal ciliate Euplotes octocarinatus centrin(N-EoCen),C-terminal EoCen(C-EoCen)and Eu^(3+)was investigated by cyclic voltammetry and pulse voltammetry using a Didodecyldimethylammonium bromide(DDAB)and agarose modified pyrolytic graphite electrode.It was found that a pair of REDOX(oxidation-reduction)peaks of the complex Eu^(3+)-N-EoCen appeared on the agarose modified electrode,which moved in a negative of 0.24 V compared with the presence of Eu^(3+)alone.However,a weak irreversible reduction peak of the complex Eu^(3+)-N-EoCen appeared on the DDAB modified electrode.The binding constantβof Eu^(3+)to N-EoCen(C-EoCen)and the binding number n could be obtained.From the intercept and slope the n=2 andβ=1.26×10^(7) L·mol^(-1) are deduced for N-EoCen,which indicated that a stable 2∶1 complex of Eu^(3+)-N-EoCen was formed.The binding ratio between C-EoCen and Eu^(3+)is 1∶2,and the binding constantβ=1.38×10^(9) L·mol^(-1).

关 键 词：中心蛋白 循环伏安 DDAB 琼脂糖 

分 类 号：O646[理学—物理化学]