FXYD6 蛋白在肝细胞癌中的表达及其临床意义  

Expression of FXYD6 protein in hepatocellular carcinoma and its clinical significance

作　　者：陈雄飞[1] 丁立爽 孔德帅[1] 赵秀雷[1] 廖丽丽 张耀敏 李凤山[1] 刘汝海[1] CHEN Xiongfei;DING Lishuang;KONG Deshuai;ZHAO Xiulei;LIAO Lili;ZHANG Yaomin;LI Fengshan;LIU Ruhai(Department of General Surgery,Cangzhou Central Hospital,Cangzhou,Hebei 061001,P.R.China)

机构地区：[1]河北省沧州市中心医院普通外科,河北沧州061001

出　　处：《中国普外基础与临床杂志》2021年第3期316-321,共6页Chinese Journal of Bases and Clinics In General Surgery

基　　金：河北省沧州市重点研发计划指导项目(项目编号:183302083)。

摘　　要：目的检测FXYD6蛋白在肝细胞癌组织及其癌旁组织中的表达,并分析其临床病理学意义。方法回顾性收集沧州市中心医院2012年3月至2018年1月期间的根治性切除的肝细胞癌蜡块80例及其癌旁组织40例,采用免疫组织化学链酶亲和素-生物素-过氧化物酶复合法(SABC)检测FXYD6蛋白的表达情况,并分析FXYD6蛋白表达与肝细胞癌患者的临床病理学特征、术后早期复发及生存时间的关系。结果FXYD6蛋白在肝细胞癌组织中的表达阳性率高于癌旁组织[77.5%(62/80)比40.0%(16/40),P<0.001]。FXYD6蛋白在肝细胞癌组织中的表达与性别、年龄、肿瘤分化程度、肿瘤最大径、肿瘤数目、术前AFP水平和HBV或HCV感染均无关(P>0.05),而与肿瘤包膜完整性、微血管侵犯和TNM分期均相关(P<0.05)。FXYD6蛋白表达阳性组的早期复发率高于阴性表达组[53.2%(33/62)比16.7%(3/18),P=0.006],然而多因素分析结果显示,FXYD6蛋白表达不是早期复发的独立危险因素(P=0.422)。FXYD6蛋白表达阳性组的术后生存情况差于阴性表达组(P=0.043),然而多因素分析结果显示,FXYD6蛋白表达不是总体生存的独立危险因素(P=0.754)。结论FXYD6蛋白在肝细胞癌组织中高表达,可能参与肝细胞的癌变及其进展,可能是肝细胞癌患者的一个不良预后因素。Objective To detect the expression of FXYD domain-containing ion transport regulator 6(FXYD6)protein in hepatocellular carcinoma tissues and the corresponding paracancerous liver tissues,and to explore the clinical significance of FXYD6 protein expression in hepatocellular carcinoma.Methods Eighty hepatocellular carcinoma tissues and the corresponding 40 paracancerous tissues were retrospectively collected in Cangzhou Central Hospital from March 2012 to January 2018,and the expression of FXYD6 protein was examined in these tissues by strept avidin-biotin complex(SABC)immunohistochemistry.We analyzed the relationship between the expression of FXYD6 protein and clinicopathological characteristics of the patients with hepatocellular carcinoma,and the relationship between the expression of the protein and early recurrence or overall survival.Results The positive expression rate of FXYD6 protein was statistically higher in hepatocellular carcinoma tissues than that in the corresponding paracancerous tissues[77.5%(62/80)vs.40.0%(16/40),P<0.001].Its expression in hepatocellular carcinoma was not related with gender,age,histological differentiation,tumor maximum diameter,tumor number,AFP concentration in serum,and HBV or HCV infection(P>0.05),but with integrity of tumor capsule,microvascular invasion,and tumor stage(P<0.05).The positive FXYD6 protein expression group had a significantly higher recurrence rate than that of the negative FXYD6 protein expression group[53.2%(33/62)vs.16.7%(3/18),P=0.006].However,multivariate analysis results showed that high FXYD6 protein expression was not a risk factor for early relapse(P=0.422).The positive FXYD6 protein expression group had a significantly shorter postoperative survival than the negative FXYD6 expression group(P=0.043).However,multivariate analysis results showed high FXYD6 protein expression was not a risk factor for overall survival(P=0.754).Conclusions FXYD6 protein was expressed abnormally in hepatocellular carcinoma tissues,which might be involved in the carcinogene

关 键 词：肝细胞癌 FXYD6蛋白 微血管侵犯 早期复发 预后 

分 类 号：R735.7[医药卫生—肿瘤]