探讨弥漫大B细胞淋巴瘤患者血浆shp1基因甲基化状态及其临床价值  

Investigatation on the methylation status of plasma shp1 gene in patients with diffuse large B-cell lymphoma(DLBCL)and its clinical value

作　　者：陈莉[1] 李青山[1] 吉慧娟 张学强[1] 张丛丛[1] 索晓慧 刘洪峰[1] 夏利敏 CHEN Li;LI Qing-shan;JI Hui-juan;ZHANG Xue-qiang;ZHANG Cong-cong;SUO Xiao-hui;LIU Hong-feng;XIA Li-min(Handan Central Hospital,Handan,Hebei 056001,China)

机构地区：[1]邯郸市中心医院血液内科,河北邯郸056001

出　　处：《中国国境卫生检疫杂志》2020年第S01期11-12,共2页Chinese Journal of Frontier Health and Quarantine

摘　　要：目的针对弥漫大B细胞淋巴瘤(DLBCL)患者采用甲基化特异性聚合酶链反应(MSP)方法检测后其血浆shpl基因的临床价值进行探讨分析。方法开展时间为2018年11月至2020年11月,研究对象为我院收治的初治弥漫大B细胞淋巴瘤患者35例以及6例良性淋巴组织增生和13例健康体检者,给予三组对象均采用甲基化特异性聚合酶链反应(MSP)方法进行检测,探讨弥漫大B细胞淋巴瘤患者血浆shp1基因甲基化状态及其临床价值。结果6例良性淋巴组织增生和13例健康体检者中均未能检测到血浆shpl基因甲基化;弥漫大B细胞淋巴瘤患者血浆中shpl基因甲基化的检出率为57.14%(20/35)、外周血白细胞中shpl基因甲基化的检出率为28.57%(10/35)、福尔马林固定石蜡包埋肿瘤组织中shpl基因甲基化的检出率为57.14%(20/35);组间对比存在统计学意义(P<0.05);shpl基因甲基化在血浆和福尔马林固定石蜡包埋肿瘤组织中具有较高的一致性。结论弥漫大B细胞淋巴瘤患者的血浆shpl基因甲基化状态可以及时反映出其肿瘤组织中的相关shpl基因甲基化特征,为临床医生的诊断和治疗提高工重要参考依据。Objective To investigate and analyze the clinical value of plasma shpl gene in patients with diffuse large B-cell lymphoma(DLBCL)using methylation-specific polymerase chain reaction(MSP)detection.Methods The period of development was from November 2018 to November 2020.The subjects of the study were 35 patients with newly diagnosed diffuse large B-cell lymphoma,6 patients with benign lymphoid tissue hyperplasia and 13 healthy patients who were admitted to our hospital,and were given three groups of subjects The methylation-specific polymerase chain reaction(MSP)method was used to detect the plasma shp1 gene methylation status and its clinical value in patients with diffuse large B-cell lymphoma.Results The methylation of shpl gene in plasma was not detected in 6 cases of benign lymphoid tissue hyperplasia and 13 cases of healthy subjects;the detection rate of shpl gene methylation in plasma of patients with diffuse large B-cell lymphoma was 57.14%(20/35).The detection rate of shpl gene methylation in peripheral blood leukocytes was 28.57%(10/35),and the detection rate of shpl gene methylation in formalin-fixed paraffin-embedded tumor tissue was 57.14%(20/35).The comparison between groups is statistically significant(P<0.05).Shpl gene methylation has a higher consistency in plasma and formalin-fixed paraffin-embedded tumor tissue.Conclusion The plasma shpl gene methylation status of patients with diffuse large B-cell lymphoma can timely reflect the relevant shpl gene methylation characteristics in their tumor tissues,which is an important reference for clinicians to improve their diagnosis and treatment.

关 键 词：弥漫大B细胞淋巴瘤 shpl基因甲基化状态 

分 类 号：R733.4[医药卫生—肿瘤]