Development of an analytical method for multi-residue quantification of 18 anthelmintics in various animal-based food products using liquid chromatography-tandem mass spectrometry  

作　　者：Kyung-Hee Yoo Da-Hee Park A.M.Abd El-Aty Seong-Kwan Kim Hae-Ni Jung Da-Hye Jeong Hee-Jung Cho Ahmet Hacimuftuoglu Jae-Han Shim Ji Hoon Jeong Ho-Chul Shin 

机构地区：[1]Department of Veterinary Pharmacology and Toxicology,College of Veterinary Medicine,Konkuk University,Seoul,143-701,Republic of Korea [2]State Key Laboratory of Biobased Material and Green Papermaking,College of Food Science and Engineering,Qilu University of Technology,Shandong Academy of Science,Jinan,250353,China [3]Department of Pharmacology,Faculty of Veterinary Medicine,Cairo University,12211,Giza,Egypt [4]Department of Medical Pharmacology,Medical Faculty,Ataturk University,Erzurum,25240,Turkey [5]Department of Veterinary Physiology,College of Veterinary Medicine,Konkuk University,Seoul,143-701,Republic of Korea [6]Natural Products Chemistry Laboratory,College of Agriculture and Life Sciences,Chonnam National University,Gwangju,500-757,Republic of Korea [7]Department of Pharmacology,College of Medicine,Chung-Ang University,Seoul,156-756,Republic of Korea

出　　处：《Journal of Pharmaceutical Analysis》2021年第1期68-76,共9页药物分析学报（英文版）

基　　金：supported by a grant(18162MFDS523)from the Ministry of Food and Drug Safety Administration in 2019.

摘　　要：In this study,we developed a simple screening procedure for the determination of 18 anthelmintics(including benzimidazoles,macrocyclic lactones,salicylanilides,substituted phenols,tetrahydropyrimidines,and imidazothiazoles)in five animal-derived food matrices(chicken muscle,pork,beef,milk,and egg)using liquid chromatography-tandem mass spectrometry.Analytes were extracted using acetonitrile/1% acetic acid(milk and egg)and acetonitrile/1% acetic acid with 0.5 mL of distilled water(chicken muscle,pork,and beef),and purified using saturated n-hexane/acetonitrile.A reversed-phase analytical column and a mobile phase consisting of(A)10 mM ammonium formate in distilled water and(B)methanol were used to achieve optimal chromatographic separation.Matrix-matched standard calibration curves(R^(2)≥0.9752)were obtained for concentration equivalent to ×1/2,×1,×2,×3,×4,and×5 fold the maximum residue limit(MRL)stipulated by the Korean Ministry of Food and Drug Safety.Recoveries of 61.2e118.4%,with relative standard deviations(RSDs)of ≤19.9%(intraday and interday),were obtained for each sample at three spiking concentrations(×1/2,×1,and ×2 the MRL values).Limits of detection,limits of quantification,and matrix effects were 0.02e5.5 mg/kg,0.06e10 mg/kg,and -98.8 to 13.9%(at 20 μg/kg),respectively.In five samples of each food matrix(chicken muscle,pork,beef,milk,and egg)purchased from large retailers in Seoul that were tested,none of the target analytes were detected.It has therefore been shown that this protocol is adaptable,accurate,and precise for the quantification of anthelmintic residues in foods of animal origin.

关 键 词：ANTHELMINTICS Tandem mass spectrometry Animal-based food products Residue analysis Method validation 

分 类 号：R155.5[医药卫生—营养与食品卫生学]