甜瓜ACS基因家族成员的鉴定及其表达特性分析  

Identification and Expression Characteristics Analysis of ACS Family Genes in Melon(Cucumis melo L.)

作　　者：赵薇 陆芳 张瑾 郝金凤[1] 哈斯阿古拉[1] Zhao Wei;Lu Fang;Zhang Jin;Hao Jinfeng;Hasi Agula(Key Laboratory of Herbage and Endemic Crop Biotechnology,School of Life Sciences,Inner Mongolia University,Hohhot,010070)

机构地区：[1]内蒙古大学生命科学学院,牧草与特色作物生物技术重点实验室,呼和浩特010070

出　　处：《分子植物育种》2021年第4期1097-1106,共10页Molecular Plant Breeding

基　　金：内蒙古自治区高等学校科学研究项目(NJZZ16016);内蒙古自然科学基金(2016MS0333)共同资助。

摘　　要：乙烯在高等植物生长发育等多个方面都具有重要的调控作用,而ACS(1-aminocyclopropane-1-carboxylic acid(ACC)synthase,1-氨基环丙烷-1羧酸合成酶)是乙烯生物合成途径的限速酶,由多基因家族编码,不同的发育、环境、激素刺激等信号诱导不同的ACS基因表达。本研究以甜瓜品种‘河套蜜瓜’(Cucumis melo L.cv.Hetao)为实验材料,应用生物信息学的方法从其基因组数据库中鉴定并筛选出ACS基因家族的全部成员,共鉴定得到11个甜瓜ACS基因。系统发生与进化关系分析结果表明,甜瓜ACS基因中,CmACS1和CmACS2属于Ⅰ型,CmACS7、CmACS10、CmACS11和CmACS12属于Ⅱ型,CmACS3和CmACS5属于Ⅲ型。启动子预测结果显示,甜瓜ACS基因启动子序列中,主要含有参与防御和胁迫响应的顺式作用元件和参与响应各种激素的顺式调控元件。半定量和实时荧光定量检测结果表明,甜瓜ACS基因表达具有组织特异性,CmACS1和CmACS11在果实跃变期表达量升高;CmACS3和CmACS5在各个组织中均未检测到表达量;CmACS7只在根中略有表达;而CmACS10和CmACS12在叶中表达量较高。这些结果预示着甜瓜ACS基因的转录调控可能参与了果实发育、成熟衰老过程以及对各种逆境的响应,这为该基因家族各成员的功能鉴定奠定了理论基础。Ethylene plays an important regulatory role in various aspects of higher plant growth and development.1-aminocyclopropane-1-carboxylic acid(ACC)synthase(ACS)is a key rate-limiting enzyme of ethylene biosynthesis pathway.It is encoded by multiple gene families.All members of the ACS gene family were identified from genome databases using bioinformatics methods in melon(Cucumis melo L.cv.Hetao).There were 11 melon ACS genes identified in melon.Phylogenetic and evolutionary relationship analysis showed that CmACS1 and CmACS2 belonged to typeⅠin melon ACS genes,CmACS7,CmACS10,CmACS11 and CmACS12 belonged to typeⅡ,and CmACS3 and CmACS5 belonged to typeⅢ.The promoter prediction results showed that the ACS genes promoter sequences mainly contained cis-acting elements involved in defense and stress responses,and cis-regulatory elements involved in response to various hormones.Semi-quantity PCR and qPCR results showed that ACS genes expression was tissue-specific,CmACS1 and CmACS11 expression levels increased during the fruit limacteric period.The expression of CmACS3 and CmACS5 was not detected in these tissues,and that of CmACS7 was detected slightly in root.CmACS10 and CmACS12 expression levels were higher in leaves.These results indicated that the transcriptional regulation of ACS genes in melon may be involved in fruit development,maturation and senescence process,and response to various kinds of stress.So these results will lay a theoretical basis for functional identification of ACS family genes in melon.

关 键 词：甜瓜 ACC合成酶 ACS基因家族 表达特性 

分 类 号：S652[农业科学—果树学]