基于重测序的芥蓝(Brassica alboglabra)全基因组InDel标记开发  

Development of InDel Markers for Brassica alboglabra Based on Whole-genome Re-sequencing Data

作　　者：洪晓如 吴智明[1] 陈汉才[2,3] 黎庭耀 沈卓[2,3] 张艳 Hong Xiaoru;Wu Zhiming;Chen Hancai;Li Tingyao;Shen Zhuo;Zhang Yan(College of Horticulture and Landscape Architecture,Zhongkai University of Agriculture and Engineering,Guangzhou,510225;Vegetable Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou,510640;Guangdong Key Laboratory for New Technology Research of Vegetables,Guangzhou,510640)

机构地区：[1]仲恺农业工程学院园艺园林学院,广州510225 [2]广东省农业科学院蔬菜研究所,广州510640 [3]广东省蔬菜新技术研究重点实验室,广州510640

出　　处：《分子植物育种》2021年第4期1190-1201,共12页Molecular Plant Breeding

基　　金：现代农业产业技术体系建设专项(CARS-25-G-36);广东省科技计划项目(2017A020208019,2018B02020-2010);广东省农作物种质资源库(圃)建设与资源收集保存、鉴评项目;广州市科技计划项目(201707010388)共同资助。

摘　　要：碱基插入/缺失(InDel)在基因组中的分布密度仅次于SNP且易于基因型分型,成为分子标记开发的主要来源。为了开发芥蓝品种间有多态性的分子标记,本研究利用2份芥蓝自交系重测序数据鉴定的InDel位点,在全基因组范围内设计了367对InDel候选标记。通过PCR检测比较8个芥蓝自交系的多态性,发现284对标记在至少2个芥蓝自交系间有多态性,阳性率为77.4%。本研究利用重测序技术开发芥蓝品种间InDel标记效率较高,为种质资源分析、基因定位、分子标记辅助育种等提供了便捷工具。Insertion and deletion(InDel)are abundant forms of genetic variation in the genome,and has been recognized as an ideal source for marker development.To provide guidance for development of polymorphic markers between cultivars in Brassica alboglabra,the whole-genome re-sequencing data of 2 Brassica alboglabra lines were applied to identify InDels.We designed 367 candidate InDel markers distributing throughout the 9 chromosomes of Chinese kale.PCR results of these candidate markers between 8 Chinese kale inbred lines revealed that 284 InDel markers displayed polymorphisms between more than two Chinese kale inbred lines.In total,the positive rate was 77.4%.In this study,the efficiency of using resequencing technology to develop InDel markers among Chinese kale varieties is high,which provides a convenient tool for germplasm resource analysis,gene mapping,and molecular marker-assisted breeding.

关 键 词：芥蓝(Brassica alboglabra) 重测序 INDEL标记 

分 类 号：S635.9[农业科学—蔬菜学]