胃癌患者血清miR-663、sHLA-G的表达及意义  

Expression of serum miR-663 and sHLA-G in patients with gastric cancer and its significance

作　　者：唐雪峰[1,2,3,4] 张蝶 张磊 TANG Xuefeng;ZHANG Die;ZHANG Lei(The Third Central Hospital of Tianjin,Tianjin 300170,China;不详)

机构地区：[1]天津市第三中心医院,天津300170 [2]天津市重症疾病体外生命支持重点实验室 [3]天津市人工细胞工程技术研究中心 [4]天津市肝胆研究所

出　　处：《山东医药》2021年第7期37-40,共4页Shandong Medical Journal

基　　金：天津市自然科学基金项目(16JCQNJC11600)。

摘　　要：目的探讨胃癌患者血清微小RNA-663(miR-663)、可溶性人类白细胞抗原-G(sHLA-G)的表达及意义。方法选取我院诊治的82例胃癌患者(胃癌组)及同时期健康体检者50例(对照组),采用qRT-PCR法检测血清中miR-663的相对表达量,采用酶联免疫吸附法(ELISA)测定血清中的sHLA-G,并分析两者在胃癌诊断中的价值。结果胃癌组血清miR-663相对表达量低于对照组,而sHLA-G水平高于对照组(P均<0.05)。相关分析结果显示,miR-663表达与胃癌发生呈负相关(r=0.922,P<0.01),sHLA-G水平与胃癌发生呈正相关(r=0.997,P<0.01)。胃癌组血清miR-663及sHLA-G表达与肿瘤TNM分期、细胞分化程度、淋巴结转移有关(P均<0.05),与性别、年龄及肿瘤直径无关(P均>0.05)。受试者工作特征(ROC)曲线分析结果显示,血清miR-663、sHLA-G诊断胃癌曲线下面积(AUC)分别为0.793、0.855。两者联合应用诊断胃癌的敏感度和特异度分别为0.878、0.860。结论胃癌患者血清miR-663表达降低、sHLA-G水平升高,且与肿瘤TNM分期、细胞分化程度、淋巴结转移有关,可作为胃癌诊断的分子标志物。Objective To investigate the expression levels of serum microRNA-663(miR-663)and soluble human leukocyte antigen-G(sHLA-G)in patients with gastric cancer and its diagnostic value.Methods Eighty-two patients with gastric cancer(gastric cancer group)diagnosed and treated in our hospital and 50 healthy controls with physical examination of the same period(control group)were selected.The expression of serum sHLA-G was measured by enzymelinked immunosorbent assay(ELISA),the relative expression of serum miR-663 was detected by real-time quantitative PCR(qRT-PCR),and their value in the diagnosis of gastric cancer was analyzed.Results The relative expression of serum miR-663 in the gastric cancer group was lower than that in the control group,while the expression of sHLA-G was higher than that in the control group,and the difference was statistically significant(P<0.05).The results of correlation analysis showed that miR-663 was negatively correlated with gastric cancer(r=-0.922,P<0.01).There was a positive correlation between sHLA-G and gastric cancer(r=0.997,P<0.01).The expression levels of serum miR-663 and sHLA-G in the gastric cancer group were related to tumor TNM stage,cell differentiation and lymph node metastasis(all P<0.05),but not to gender,age or tumor diameter(all P>0.05).Receiver operating characteristic(ROC)curve analysis results showed the relative expression of serum miR-663 and the expression level of sHLA-G had higher diagnostic value,and the area under the curve(AUC)was 0.793 and 0.855,respectively.The diagnostic efficacy of the combination of the two was higher,and the sensitivity was 0.878 and the specificity was 0.860.Conclusion The expression of serum miR-663 decreases and the level of sHLA-G increases in patients with gastric cancer,which are related to tumor TNM stage,cell differentiation and lymph node metastasis,and both of them can be used as the molecular markers for the diagnosis of gastric cancer.

关 键 词：胃癌 微小RNA-663 可溶性人类白细胞抗原-G 诊断价值 

分 类 号：R735.2[医药卫生—肿瘤]