异普黄酮对大鼠正畸牙复发过程中牙周组织改建的影响  

Effect of ipriflavone on reconstruction of periodontal tissues during recurrence of orthodontic teeth in rats

作　　者：代书林 王旭霞 乜福娇 崔群 韩媛媛 张君 DAI Shu-lin;WANG Xu-xia;NIE Fu-jiao;CUI Qun;HAN Yuan-yuan;ZHANG Jun(Department of Orthodontics,School and Hospital of Stomatology,Cheeloo College of Medicine,Shandong University,Jinan 250012;Shandong Key Laboratory of Oral Tissue Regeneration&Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration,Jinan 250012;Department of Stomatology,Guanxian Central Hospital,Guanxian 252500;Department of Oral and Maxillofacial Surgery,School and Hospital of Stomatology,Cheeloo College of Medicine,Shandong University,Jinan 250012,Shcuidong Province,China)

机构地区：[1]山东大学齐鲁医学院口腔医学院·口腔医院口腔正畸科,山东济南250012 [2]山东省口腔组织再生重点实验室,山东省口腔生物材料与组织再生工程实验室,山东济南250012 [3]冠县中心医院口腔科,山东冠县252500 [4]山东大学齐鲁医学院口腔医学院·口腔医院口腔颌面外科,山东济南250012

出　　处：《上海口腔医学》2021年第1期23-27,共5页Shanghai Journal of Stomatology

基　　金：山东省自然科学基金(ZR2019MH113)。

摘　　要：目的:探讨异普黄酮对大鼠正畸牙移动复发过程中牙周组织改建的影响。方法:选择8周龄雄性SD大鼠24只,随机分为2组,即异普黄酮组(IP组)和对照组,每组12只。首先建立大鼠正畸牙移动后复发模型,连续加力10 d后去除加力装置。加力装置移除后,IP组给予10 mg/(kg·d)异普黄酮灌胃,对照组给予等量生理盐水灌胃。给药后0、1、3、7、10 d,局麻下制备2组大鼠上颌印模,灌注石膏模型,测量、评估复发距离。药物灌注10 d后,收集组织标本,H-E染色观察牙周组织改建,免疫组织化学染色观察骨形态发生蛋白2(BMP-2)的表达。利用Image-Pro 6.0软件分析染色切片吸光度值,采用SPSS 22.0软件包对数据进行统计学分析。结果:拆除矫治器后10 d内,2组正畸牙移动均有明显复发,IP组的复发距离显著小于对照组,第10天时仍显著小于对照组。H-E染色和免疫组织化学染色结果显示,与体内对照组相比,IP组的牙周组织中有更多的新骨形成和更多的BMP-2表达。结论:在正畸牙移动复发过程中,异普黄酮能促进牙周组织中BMP-2的表达,促进牙周组织骨改建,有效降低正畸牙移动复发速率。PURPOSE:To investigate the effect of ipriflavone on reconstruction of periodontal tissues during recurrence of orthodontic teeth.METHODS:Twenty-four male SD rats were randomized into 2 groups,ipriflavone group(IP group)and control group,there were 12 rats in each group.The model of recurrence after movement of orthodontic teeth in rats was established.After continuous loading for 10 days,the loading devices were removed.Rats in ipriflavone group(IP group)were given ipriflavone intragastrically for 10 mg/(kg·d)after the devices were removed,while rats in the control group were given an equivalent amount of normal saline after the devices were removed.On the 0 th,1 st,3 rd,7 th,and 10 th day of administration,the rat maxillary impression and plaster model of two groups were prepared under local anesthesia,the distance between maxillary first molar lingual sulcus point and third molar in lingual groove point was measured to evaluate the relapse distance.After drug infusion for 10 days,the collected tissue specimens were stained with H-E to observe periodontal reconstruction,and expression of bone morphogenetic protein-2(BMP-2)was detected by immunohistochemical staining.Software Image-Pro 6.0 was used to analyze the optical density values of the stained sections.The data were analyzed with SPSS 22.0 software package.RESULTS:After removing the orthopaedic devices for10 days,there was a significant recurrence of the movement of the orthodontic teeth in both groups.The recurrent distance of IP group was significantly smaller than that of the control group,and still significantly smaller than that of the control group at 10 d.H-E staining and immunohistochemical staining results showed that the IP group had more new bone formation and more BMP-2 expression in the periodontal tissues compared to the control group in vivo.CONCLUSIONS:In the process of recurrence of orthodontic tooth movement,ipriflavone can promote the expression of BMP-2 in periodontal tissue,improve bone remodeling of periodontal tissue,and effectively

关 键 词：异普黄酮 正畸治疗 复发 BMP-2 

分 类 号：R783.5[医药卫生—口腔医学]