3D打印HAP-GEL支架复合BMSCs和HUVECs修复兔颅骨缺损的效果评价  

Evaluation of the effect of 3D printed HAP-GEL scaffold combined with BMSCs and HUVECs in repairing rabbit skull defect

作　　者：王宏 范海霞 程焕芝 李然 郭秀娟[3] 耿海霞 WANG Hong;FAN Hai-xia;CHENG Huan-zhi;LI Ran;GUO Xiu-juan;GENG Hai-xia(Department of Stomatology,Weifang Medical University,Weifang 261053;Department of Stomatology,Jining Medical University,Jining 272000;Department of Stomatology,Affiliated Hospital of Jining Medical University,Jining 272000,Shandong Province,China)

机构地区：[1]潍坊医学院口腔医学院,山东潍坊261053 [2]济宁医学院口腔医学院,山东济宁272000 [3]济宁医学院附属医院口腔科,山东济宁272000

出　　处：《上海口腔医学》2021年第1期28-32,共5页Shanghai Journal of Stomatology

基　　金：山东省自然科学基金(ZR2017LH044);济宁医学院国家自然科学基金培育项目(2016-09);济宁医学院教师科研扶持项目(JYFC2018KJ018)。

摘　　要：目的:定性分析3D打印羟基磷灰石-胶原(hydroxyapatite-gelatin,HAP-GEL)支架材料复合骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)和脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)修复兔颅骨缺损的效果。方法:将第3代BMSCs和HUVECs与3D打印HAP-GEL支架材料共培养,构建组织工程骨。建立兔颅骨缺损模型,随机分为4组,分别植入HAP-GEL支架、HAP-GEL支架+BMSCs和HUVECs细胞,并设置阳性对照组(自体骨组织)和空白对照组。术后12周行X线片、锥形束CT(CBCT)扫描、H-E染色,定性分析骨缺损修复情况。结果:术后12周,影像学检测(X线和CBCT)显示,空白对照组仍有明显圆形透射影;HAP-GEL支架复合细胞组与HAP-GEL组均密度增高,缺损边界模糊,其中,HAP-GEL支架复合细胞组骨质连续,骨密度最高,接近正常组织。H-E染色结果显示,与空白对照组及HAP-GEL组相比,HAP-GEL复合材料组缺损区域被新生骨及骨样组织充填,支架材料有所降解且支架内部已有新骨生成,骨修复效果良好,成骨效果与阳性对照组相似。结论:3D打印HAPGEL支架+BMSCs+HUVECs细胞复合体具备良好的成骨能力和生物相容性,对兔颅骨缺损具有较好的修复作用。PURPOSE:To qualitatively analyze the effect of 3 D printed hydroxyapatite-gel(HAP-GEL)scaffold combined with bone marrow mesenchymal stem cells(BMSCs)and human umbilical vein endothelial cells(HUVECs)in repairing rabbit skull defect.METHODS:The third generation BMSCs and HUVECs were co-cultured with 3 D printed HAP-GEL scaffold to construct tissue engineering bone.The rabbit model of skull defect was established and randomly divided into 4 groups.HAP-GEL stent,HAP-GEL stent+BMSCs and HUVECs cells were implanted respectively,and positive control(autologous bone tissue)and blank control were set up.Twelve weeks after operation,X-ray,cone-beam CT(CBCT)scan and H-E staining were performed to observe and analyze the changes of bone defect qualitatively.RESULTS:Twelve weeks after operation,imaging examination(X-ray and CBCT)showed that there was still obvious circular transmission in the blank control group,and the density was increased and the defect boundary was blurred in both HAP-GEL stent combined cell group and HAP-GEL group,among which the bone was continuous and the bone mineral density was the highest in HAP-GEL stent composite cell group,which was close to normal tissue.The results of H-E staining at twelve weeks showed that compared with the blank control group and the HAP-GEL group,the defect area of the HAP-GEL composite group was filled with new bone and bone-like tissue,the scaffold material was degraded and there was new bone formation inside the scaffold,and the bone repair effect was good,and the osteogenic effect was similar to that of the positive control group.CONCLUSIONS:3 D printed HAP-GEL scaffold+BMSCs+HUVECs cell complex has good osteogenic ability and biocompatibility with a good effect on repairing rabbit skull defect.

关 键 词：组织工程骨 3D打印 羟基磷灰石 骨髓间充质干细胞 脐静脉内皮细胞 

分 类 号：R782.2[医药卫生—口腔医学]