水产品及其制品中大西洋鲑鱼实时荧光PCR检测方法建立  

Detection Method Construction by Real-Time PCR for Salmosalar in Aquatic Product and Its Processed Products

作　　者：王坤[1] 林霖[1] 吴佳辉[1] 朱成杰 余灏 张世伟[1] 杨国武[1] WANG Kun;LIN Lin;WU Jiahui;ZHU Chenjie;YU Hao;ZHANG Shiwei;YANG Guowu(Shenzhen Academy of Metrology&Quality Inspection,Shenzhen 518131,China)

机构地区：[1]深圳市计量质量检测研究院,广东深圳518131

出　　处：《食品科技》2021年第1期298-302,共5页Food Science and Technology

基　　金：深圳市食品安全战略项目(JSGG20140519114050315-7)。

摘　　要：目的:三文鱼营养丰富,含有ω-3不饱和脂肪酸,深受大众喜爱,但存在产品标签标示错误、掺伪掺假、以次充好的现象。目前采用检测标准SN/T 3589.7—2013《出口食品中常见鱼类及其制品的鉴伪方法第七部》对鲑鱼成分检测,无法区分大西洋鲑鱼和其他种类的三文鱼。因此有必要建立一种用于区分上述几种三文鱼的快速检测方法。方法:研究建立了针对大西洋鲑鱼成分鉴定的实时荧光PCR法,并对该方法的特异性和检出限进行了验证。结果:该方法具有良好的特异性,检出限为1%(w/w)。结论:可应用于大西洋鲑鱼的日常检测,为监管部门提供技术支持。Objective:Salmon is rich in nutrition and contains omega-3 unsaturated fatty acids,which is popular among the public.However,there are some problems such as wrong labeling,adulteration and inferior quality.The current detection standard SN/T 3589.7—2013 can not distinguish Salmosalar from other salmon.It is necessary to establish a rapid detection method for distingnishing the above-mentioned types of salmon.Method:In this study,a real-time PCR method for the identification of Salmosalar was established,and its specificity and detection limit were verified.Result:It shows that the method has good specificity,detection limit is 1% (w/w).Conclusion:It can be applied to the daily detection of Salmosalar,provide technical support for the supervision department.

关 键 词：大西洋鲑 属性鉴别 实时荧光PCR 

分 类 号：TS207.3[轻工技术与工程—食品科学]