鄂西北地区食源性金黄色葡萄球菌污染及耐药性和毒力基因分析  

Analysis of resistance and virulence genes of foodborne Staphylococcus aureus in northwestern Hubei Province

作　　者：赵朵 裴曼君 张文乐 梁冉 肖娜 唐一通 ZHAO Duo;PEI Manjun;ZHANG Wenle;LIANG Ran;XIAO Na;TANG Yitong(Medicine College,Hubei University of Arts and Science,Hubei Xiangyang 441053,China)

机构地区：[1]湖北文理学院医学院,湖北襄阳441053

出　　处：《中国食品卫生杂志》2020年第6期620-625,共6页Chinese Journal of Food Hygiene

基　　金：襄阳市科技研究与开发项目[襄科计(2017)10号];湖北省自然科学基金项目(2016CFB319);湖北文理学院青年学者建设基金(F614)。

摘　　要：目的了解鄂西北地区食源性金黄色葡萄球菌的检出情况、耐药性以及毒力基因分布特征。方法从湖北省襄阳市、十堰市、随州市三地共采集303份食物样品进行金黄色葡萄球菌筛查,通过聚合酶链式反应(PCR)法进行耐甲氧西林金黄色葡萄球菌(methicillin-resistant Staphylococcus aureus,MRSA)的鉴定和毒力基因检测,并用K-B纸片扩散法进行耐药性测定。结果303份样品中共有41份检出金黄色葡萄球菌,阳性率为13.53%,其中生肉制品检出率最高(23.91%,22/92)。在产肠毒素菌株中以携带产肠毒素基因sea的菌株最多,占87.80%(36/41)。携带表皮剥脱素基因eta和中毒性休克综合征毒素基因tst的菌株分别占97.56%(40/41)和7.32%(3/41)。同时携带3种及以上肠毒素基因的菌株占17.07%(7/41)。同时含有eta和tst的菌株占2.44%(1/41)。药敏结果显示,分离菌株对青霉素、四环素、红霉素和多西环素的耐药率分别为78.05%(32/41)、43.90%(18/41)、31.71%(13/41)和21.95%(9/41),对其他8种抗菌药物的耐药率均<20%。mecA基因检测表明,19.51%(8/41)的菌株为MRSA,且7株MRSA菌株来源于生肉制品和未加调料的素卤菜。结论鄂西北地区食源性金黄色葡萄球菌具有较高的检出率,而且产毒株较多,并且存在多重耐药现象,相关部门需加强食品安全监测以控制该菌株的流行与扩散。Objective To understand the prevalence,drug resistance,and virulence gene distribution of foodborne Staphylococcus aureus in northwestern Hubei Province.Methods A total of 303 food samples were collected from Xiangyang City,Shiyan City,and Suizhou City of Hubei Province for Staphylococcus aureus screening.Toxic genes and methicillin-resistant Staphylococcus aureus(MRSA)were detected with PCR method.The drug resistance of Staphylococcus aureus was determined with K-B paper diffusion method.Results Staphylococcus aureus strain was determined from 41 samples with the positive rate of 13.53%.Among them,the highest detection rate was from raw meat products(23.91%,22/92).Among the enterotoxigenic strains,the strain carrying sea was the most common,accounting for 87.80%(36/41).The strains carrying eta and tst accounted for 97.56%(40/41)and 7.32%(3/41),respectively.Strains carrying three or more enterotoxin genes accounted for 17.07%(7/41).2.44%(1/41)of strains carry eta and tst simultaneously.The drug susceptibility result showed that the penicillin,tetracycline,erythromycin,and doxycycline accounted for 78.05%(32/41),43.90%(18/41),31.71%(13/41)and 21.95%(9/41)respectively.The mecA gene test showed that 19.51%(8/41)of the strains were MRSA strains.Conclusion Foodborne Staphylococcus aureus in northwestern Hubei Province had a higher detection rate,toxic gene carring rate,and multiple drug resistance.Related departments need to strengthen food safety monitoring to control the spread of the strain.

关 键 词：金黄色葡萄球菌 耐药性 毒力基因 食品安全 

分 类 号：R155[医药卫生—营养与食品卫生学]