蜡梅科6种植物嫩梢挥发性成分的HS–SPME–GC–MS分析  

HS–SPME–GC–MS Analysis of Volatile Components in Tender Shoots from Six Plants of Calycanthaceae

作　　者：沈植国 孙萌 袁德义[1] 程建明 丁鑫[2] 尚忠海 SHEN Zhiguo;SUN Meng;YUAN Deyi;CHENG Jianming;DING Xin;SHANG Zhonghai(College of Forestry,Central South University of Forestry and Technology Changsha 410004,China;Henan Academy of Forestry,Zhengzhou 450008,China;Henan Kalefu Horticulture Limited Company Zhengzhou 450000,China)

机构地区：[1]中南林业科技大学林学院,长沙410004 [2]河南省林业科学研究院,郑州450008 [3]河南卡乐夫园艺有限公司,郑州450000

出　　处：《园艺学报》2020年第12期2349-2361,共13页Acta Horticulturae Sinica

基　　金：河南省科技兴林项目(豫财环资[2019]35号);许昌英才计划创新团队项目(许文[2018]26号)。

摘　　要：采用顶空固相微萃取(HS–SPME)与气质联用(GC–MS)分析技术,对蜡梅科6种植物挥发性成分进行检测,并通过共有成分分析、主成分分析、聚类分析,与药用价值较高的山蜡梅主要挥发性成分进行对比。检测结果表明:蜡梅‘鸿运’(Chimonanthus praecox‘Hongyun’)、‘金玉’(Ch. praecox‘Jinyu’)和‘鄢陵素心’(Ch.praecox‘Yanlingsuxin’),山蜡梅(Ch.nitens)、夏蜡梅(Calycanthus chinensis)及美国蜡梅(Ca. floridus)嫩梢挥发性成分种类分别为47、48、44、44、48和46种,共包含141种成分;蜡梅科6种植物共有挥发性成分以烯烃类、醇类物质为主,‘鸿运’‘金玉’‘鄢陵素心’蜡梅、夏蜡梅和美国蜡梅与山蜡梅共有挥发成分分别为15、18、10、14和20种;PCA分析和聚类分析结果表明:夏蜡梅单独为一类,‘鸿运’‘金玉’‘鄢陵素心’蜡梅为一类,山蜡梅和美国蜡梅为一类;‘鸿运’‘金玉’‘鄢陵素心’蜡梅、夏蜡梅和美国蜡梅与山蜡梅的主要挥发性成分对比分析表明:桉叶油醇、对薄荷–1(7),3–二烯为主要差异成分。以上结果表明,6个蜡梅科植物资源嫩梢挥发性成分组成丰富,但种(品系)间存在一定的差异,均具有较大的药用开发潜力。The headspace solid phase microextraction(HS–SPME)combained with the GC–MS test were used to detect the volatile components of six Calycanthaceae plants. Moreover,in order to investigate the volatile components difference of the tender shoots between several species(strains)of Calycanthaceae,the common component analysis,principal component analysis and cluster analysis of the volatile components were adopted to compare the Chimonanthus nitens(a widely used medicinal plant)with other tested species(strains). The results showed that a total of 141 volatile components were detected in the tender shoots, with 47 in Ch. praecox‘Hongyun’,48 in Ch. praecox‘Jinyu’,44 in Ch. praecox‘Yanlingsuxin’,44 in Ch. nitens,48 in Calycanthus chinensis and 46 in Ca. floridus. Alkenes and alcohols were the main volatile components in the six plants. Moreover,when compared with Ch. nitens,the common volatile components of Ch. praecox‘Hongyun’,‘Jinyu’,‘Yanlingsuxin’,Ca. chinensis,and Ca. floridus were 15,18,10,14 and 20,respectively. PCA analysis and cluster analysis showed that Ca. chinensis falled into a group,while Ch. nitens and Ch. floridus belong to one group,and Ch. praecox‘Hongyun’,‘Jinyu’and‘Yanlingsuxin’are the other one. Furthermore,we found eudesmol and β-terpinene were the primary differential volatile compounds when executing the comparative analysis with Ch. nitens. Overall, abundant volatile components were found in tender shoots of the six Calycanthaceae plants,and our study have great potential for medical development though there are certain discrepancy among different species(strains).

关 键 词：蜡梅科 嫩梢 挥发性成分 顶空固相微萃取技术(HS–SPME) 气质联用(GC–MS) 

分 类 号：S685.17[农业科学—观赏园艺]