黄脚胡蜂提取物对HepG2人肝癌细胞的抑制作用  

Inhibitory Effects of Vespa velutina Extract on HepG2 Human Hepatoma Cells

作　　者：杨巧玲 赵若丹 刘晓波 郭美仙 陈旭冰 Yang Qiaoling;Zhao Ruodan;Liu Xiaobo;Guo Meixian;Chen Xubing(Yunnan Provincal Key Laboratory of Entomological Biopharmaceutical R&D,Dali,Yunnan 671000,China;College of Pharmacy,Dali University,Dali,Yunnan 671000,China)

机构地区：[1]云南省昆虫生物医药研发重点实验室,云南大理671000 [2]大理大学药学院,云南大理671000

出　　处：《大理大学学报》2021年第2期34-38,共5页Journal of Dali University

基　　金：云南省教育厅科学研究基金项目(2020J0568);中国西南药用昆虫及蛛形类资源开发利用协同创新中心2018年度开放课题(CIC1806)。

摘　　要：目的:研究黄脚胡蜂提取物对HepG2人肝癌细胞增殖的影响。方法:以不同浓度的黄脚胡蜂提取物作用于体外培养的HepG2人肝癌细胞,MTT法检测吸光度,计算细胞抑制率。结果:1000.0μdg/mL的黄脚胡蜂乙酸乙酯层提取物(HJ2)、正丁醇层提取物(HJ3)、水层提取物(HJ4)和黄脚胡蜂乙醇总提取物(HJ5)对HepG2人肝癌细胞的抑制率分别为60.96%、62.95%、62.39%和65.98%,且抑制作用随浓度的降低而减弱;黄脚胡蜂HJ2、HJ3、HJ4和HJ5的半数抑制浓度分别为211.8、173.6、124.0和165.1μg/mL。结论:黄脚胡蜂提取物对HepG2人肝癌细胞的体外生长均有一定程度的抑制作用,其中黄脚胡蜂水层提取物和乙醇总提取物效果较好。Objective:To study the effects of Vespa velutina extract on the proliferation of HepG2 human hepatoma cells.Methods:Vespa velutina extract of different concentrations were applied to HepG2 human hepatoma cells cultured in vitro,and MTT assay was used to detect the absorbance value and calculate the cell inhibition rate.Results:The inhibition rates of ethyl acetate layer extract(HJ2),n-butanol layer extract(HJ3),water layer extract(HJ4)and total ethanol extract(HJ5)of 1000.0μg/mL of Vespa velutina on HepG2 human hepatoma cells were 60.96%,62.95%,62.39%and 65.98%respectively,and the inhibitory effects decreased with the decrease of concentration;the half inhibition concentrations of HJ2,HJ3,HJ4 and HJ5 of Vespa velutina were 211.8,173.6,124.0 and 165.1μg/mL,respectively.Conclusion:Vespa velutina extract has a certain inhibition effect on the growth of HepG2 human hepatoma cells in vitro,and there is a dose-dependent effect,among which the water layer and total ethanol extract of Vespa velutina were better.

关 键 词：黄脚胡蜂 肝癌细胞 MTT法 抑制作用 

分 类 号：R735.7[医药卫生—肿瘤]