青海省不同地区羌活挥发性成分AOE含量的测定  

Determination of AOE Content of Volatile Components of Notopterygium Incisum Ting ex H.T.Chang in Different Regions of Qinghai Province

作　　者：李容榕 盛观星 林小艳 LI Rong-rong;SHENG Guan-xing;LIN Xiao-yan(School of Pharmacy,Qinghai Nationalities University,Xining Qinghai,810007;Key Laboratory of Phytochemistry of Qinghai Tibet Plateau,Xining Qinghai,810007)

机构地区：[1]青海民族大学药学院,青海西宁810007 [2]青海省青藏高原植物化学重点实验室,青海西宁810007

出　　处：《山西大同大学学报（自然科学版）》2021年第1期8-10,15,共4页Journal of Shanxi Datong University(Natural Science Edition)

摘　　要：采用植物抗氧化酶酶联免疫分析方法测定青海省互助北山林场、大通县、祁连县4种羌活挥发性成分的AOE含量。结果发现,水蒸气蒸馏法羌活挥发性成分AOE含量有明显地区差异,互助北山林场的羌活AOE含量最高,达9.99 U/g,大通县的羌活AOE含量最低,为5.06 U/g。超临界CO2萃取得青海省不同地区羌活挥发性成分的AOE含量无明显差异,均低于4.00 U/g。结论:不同提取方法对植物抗氧化酶提取有较大影响,同时AOE含量可以较好地反映出不同地区羌活抗氧化活性的强弱。The AOE content of volatile constituents was determined by using plant antioxidant enzyme-linked immunosorbent assay(ELISA)method in four different species of Notopterygium incisum Ting ex H.T.Chang in Qinghai Province,Huzhu Beishan Forest Farm,Datong County of Qinghai Province and Qilian County of Qinghai Province.Results:There was a significant regional difference in the content of volatile AOE in steam distillation.The AOE content of Huzhu Beishan Forest Farm in Qinghai Province was the highest,reaching 9.99 U/g.The AOE content of Datong County in Qinghai Province was the lowest,being 5.06 U/g.Supercritical CO2 extraction showed no significant difference in AOE content of volatile components in different regions of Qinghai Province,all lower than 4.00 U/g.Conclusion:Different extraction methods have great influence on the extraction of antioxidant enzymes in plants.At the same time,AOE content can better reflect the strength of antioxidant activity in different regions.

关 键 词：羌活 挥发性成分 AOE 含量测定 

分 类 号：Q946.8[生物学—植物学]