酶法提取淡水鱼内脏鱼油的工艺优化  

Optimization of Enzymatic Extraction of Offal Oil from Fresh Water Fish

作　　者：顾盼祺 魏晓倩 赵利 陈丽丽 江勇 白春清 袁美兰 GU Pan-qi;WEI Xiao-qian;ZHAO Li;CHEN Li-li;JIANG Yong;BAI Chun-qing;YUAN Mei-lan(National R&D Branch Center of Fresh Water Fish Processing Technology(Nanchang),College of Life Science,Jiangxi Normal University of Science and Technology,Nanchang 330013,China)

机构地区：[1]江西科技师范大学生命科学学院,国家淡水鱼加工技术研发分中心(南昌),南昌330013

出　　处：《中国调味品》2021年第3期95-99,共5页China Condiment

基　　金：江西省科技厅科技支撑重大项目(20152ACF60008);江西省教育厅科技项目(GJJ150820);江西省现代农业产业技术体系建设专项资金资助(赣财教指2013-258);国家农业科技成果转化项目(国科办农[2014]42号)。

摘　　要：以淡水鱼内脏为原料,以鱼油得率为评价指标,对酶法提取鱼油的工艺条件进行优化。首先采用木瓜蛋白酶,中性蛋白酶和碱性蛋白酶在各自的最佳酶活条件下提取内脏鱼油,得到最佳的水解用酶为中性蛋白酶。然后以中性蛋白酶为水解用酶,在单因素试验基础上,采用正交试验设计,分别考察pH值、中性蛋白酶添加量、酶解温度、酶解时间和料液比对鱼油得率的影响,最终得到中性蛋白酶酶解法提取鱼油的最优条件为:pH值7,酶解时间40 min,酶解温度60℃,酶添加量0.50%,料液比(原料∶蒸馏水)1∶1.5。在最优条件下,鱼油得率可达35.67%。With fresh water fish offal as the raw material and fish oil yield as the evaluation index,the extraction conditions of fish oil by enzymatic method are optimized.Firstly,papain,neutral protease and alkaline protease are used to extract offal fish oil under their respective optimal enzyme activity conditions,and the optimal hydrolysis enzyme is neutral protease.Secondly,the neutral protease is used as the hydrolysis enzyme,the orthogonal experiment design is adopted to investigate the effects of pH value,additive amount of neutral protease,enzymatic hydrolysis temperature,enzymatic hydrolysis time and solid-liquid ratio on the yield of fish oil based on the single factor experiment.Ultimately,the optimal conditions for extracting fish oil by neutral protease hydrolysis are as follows:pH value is 7,enzymatic hydrolysis time is 40 min,enzymatic hydrolysis temperature is 60℃,enzyme additive amount is 0.50%,the ratio of solid to liquid(raw material∶distilled water)is 1∶1.5.Under the optimal conditions,the yield of fish oil can reach 35.67%.

关 键 词：酶法 淡水鱼 内脏 提取鱼油 工艺 

分 类 号：TS222.3[轻工技术与工程—粮食、油脂及植物蛋白工程]