GGT在小鼠皮肤成纤维细胞中的表达及抑制酶活性对COL-Ⅰ表达的影响  

GGT is expressed in mouse skin fibroblast and GGT inhibitory activities is involved in the upregulation of CollagenⅠexpression in the mouse skin fibroblast

作　　者：姜瑛 王祥 张蕾 常怀广 Jiang Ying;Wang Xiang;Zhang Lei;Chang Huai-guang(HaiShu Dentistry Department of Stomatological,NingBoZheJiang 315000,China;NingBo Vocational College of Health,Department of Stomatology,NingBo ZheJiang 315000,China)

机构地区：[1]海曙牙科医院口腔科,浙江宁波315000 [2]宁波卫生职业技术学院口腔医学教研室,浙江宁波315100

出　　处：《医学食疗与健康》2021年第5期14-16,20,共4页Medical Diet and Health

基　　金：宁波市自然科学基金资助项目(编号:202003N4187)。

摘　　要：目的:观察γ-谷酰基转移酶(gamma-glutamyl transferase,GGT)在小鼠皮肤成纤维细胞中的表达,探究抑制其活性后对细胞存活率及Ⅰ型胶原表达的影响。方法:胰酶、胶原酶两步消化法分离新生C57BL/6小鼠皮肤成纤维细胞并在培养板中培养至稳定贴壁生长;免疫组化方法检测GGT在小鼠皮肤成纤维细胞中的表达;生长噻唑蓝(MTT)法检测不同浓度GGsTOP对体外培养小鼠皮肤成纤维细胞存活率的影响;免疫荧光观察COL-Ⅰ的表达。结果:GGT在小鼠皮肤成纤维细胞中广泛表达。抑制剂GGsTOP可以促进小鼠皮肤成纤维细胞增殖,并且使细胞内Ⅰ型-胶原(type Ⅰ collagen,COL-Ⅰ)蛋白水平的表达增强。结论:GGT在小鼠皮肤成纤维细胞中广泛表达,抑制GGT酶活性后促进Ⅰ型-胶原表达,提示抑制剂GGsTOP可能促进创面愈合再上皮化过程。Objective:To observe the gamma glutamyl transferase(GGT)expression on mouse skin fibroblasts(MSF),and explore whether inhibitory activity of GGT influence cell survival and collagen typeⅠ(COL-1)expression.Methods:Primary mouse dermal fibroblast cells were isolated from neonatal C57BL/6 mice by trypsin-collagen two-step digestion.The expression of GGT was analyzed by immunohistochemistry,and the effects of GGsTOP on proliferation of mouse skin fibroblasts cells were evaluated by MTT assay.The collagen typeⅠexpression wasmeasured by immunofluorescence.Results:GGT was widely expressed on mouse skin fibroblasts.Inhibitors GGsTOP promoted mouse skin fibroblasts proliferation,and immunofluorescence showed that the protein expressions of COL-1were up-regulated.Conclusion:GGT was widely expressed in mouse skin fibroblast cells,inhibitors GGsTOP was related to the up-regulation of the expression of COL-1 while inhibition activity of GGT,inhibitors GGsTOP may promote on the re-epithelialization process of wound healing.

关 键 词：γ-谷酰基转移酶 皮肤成纤维细胞 细胞增殖 Ⅰ型-胶原 再上皮化 

分 类 号：R363[医药卫生—病理学]