联氨氧化酶初步纯化及醌类化合物对其活性影响  

Preliminary purification of hydrazine oxidizing enzyme and effect of quinones on its activity

作　　者：李竺芯 张寿通[1] 李阳 阎松[1] LI Zhuxin;ZHANG Shoutong;LI Yang;YAN Song(School of Environment and Chemical Engineering, Dalian Jiaotong University, Dalian 116021, China)

机构地区：[1]大连交通大学环境与化学工程学院,辽宁大连116021

出　　处：《大连理工大学学报》2021年第2期136-142,共7页Journal of Dalian University of Technology

基　　金：国家自然科学基金资助项目(51408094).

摘　　要：厌氧氨氧化菌是一种化能自养菌,它具有独特的生理生化特性及生物脱氮机理.对厌氧氨氧化菌进行实验室扩大培养,对其主要代谢酶联氨氧化酶性质进行研究,对联氨氧化酶进行初步纯化,并探究醌类化合物对联氨氧化酶活性的影响.最终得出联氨氧化酶活性最适温度为35℃,最适pH为7.5.酶纯化结果表明超滤相对分子质量在50~100 kDa.投加辅酶Q对提高酶活性效果最好,2-羟基-1,4-萘醌对酶活性提高效果稍差,蒽醌-2-磺酸钠盐对酶活性基本无影响.AnAOB is a kind of chemoautotrophic bacteria,which has unique physiological and biochemical characteristics and biological denitrification mechanism.The laboratory expanding culture of AnAOB is conducted,the property of its main metabolic enzyme-hydrazine oxidizing enzyme(HZO)is studied,the preliminary purification of HZO is carried out,and the effect of quinones on the activity of HZO is discussed.It is found that the optimum enzyme activity temperature of HZO is 35℃,the optimum pH is 7.5.Enzyme purification result shows that the relative molecular mass of ultrafiltration is 50-100 kDa.Coenzyme Q has the best effect on improving enzyme activity,2-hydroxy-1,4-naphthoquinone has a less effect on enzyme activity improvement,and anthraquinone-2-sulfonate has no effect on enzyme activity.

关 键 词：厌氧氨氧化菌 联氨氧化酶 纯化 醌类化合物 酶活性 

分 类 号：X172[环境科学与工程—环境科学]