原发性肝癌中P38γ高表达及其对肿瘤形成作用  被引量:2

P38γOverexpression in Primary Liver Cancer and Its Effect on Tumor Formation

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作  者:陈翔[1] 胡晟[1] 周玉庆[1] 高宏 吴林 赵伟 张璠 CHEN Xiang;HU Sheng;ZHOU Yuqing(Chongqing Emergency Medical Center,Chongqing,400014)

机构地区:[1]重庆市急救医疗中心,400014

出  处:《实用癌症杂志》2021年第2期206-209,共4页The Practical Journal of Cancer

摘  要:目的分析肝癌患者中P38γ表达量,并通过干扰P38γ表达分析其对肝癌肿瘤形成的影响。方法通过Western-Blot检测肝癌患者组织中P38γ的表达水平,再构建P38γ干扰质粒Si-P38γ,将Si-P38γ转染Hep3B细胞,检测其对P38γ蛋的影响。最后,通过Hep3B裸鼠成瘤实验分析Si-P38γ在裸鼠体内对肿瘤形成的影响。结果肝癌患者组织中P38γ表达量显著高于正常组织;通过Si-P38γ质粒转染可以降低P38γ表达,转染36小时后P38γ基本不表达。Hep3B裸鼠成瘤实验证明,Si-P38γ干扰可以显著降低Hep3B成瘤数,同时肿瘤体积显著降低。结论干扰P38γ表达可以影响肝癌的形成,其有望成为治疗肝癌的新靶点。Objective To analyze the expression of P38γin hepatocellular carcinoma patients and analyze its effect on the formation of hepatocarcinoma by interfering with the expression of P38γ.Methods The expression of P38γin teratocarcinoma tissues was detected by Western-Blot.The P38γinterference plasmid Si-P38γwas constructed and Si-P38γwas transfected into Hep3B cells to detect its effect on P38γeggs.Finally,the effect of Si-P38γon tumor formation in nude mice was analyzed by Hep3B nude mice.Results The expression of P38γin liver cancer patients was significantly higher than that in normal tissues.The expression of P38γwas decreased by Si-P38γplasmid transfection,and P38γwas not expressed after 36 hours of transfection.The tumor formation experiment of Hep3B nude mice demonstrated that Si-P38γinterference can significantly reduce the number of Hep3B tumor formation,and the tumor volume is significantly reduced.Conclusion Interfering with P38γexpression may affect the formation of liver cancer,which is expected to become a new target for the treatment of liver cancer.

关 键 词:P38γ 肝癌 干扰 成瘤 

分 类 号:R735.7[医药卫生—肿瘤]

 

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