血清lncRNA HULC水平与乙型肝炎病毒相关性肝细胞癌患者临床特征及预后的关系  

Relations of serum lncRNA HULC level with the clinical features and prognosis of patients with hepatitis B virus-related hepatocellular carcinoma

作　　者：李俊[1] 李理[1] 李勇敢 赵延兵[1] 张玮[1] 苏瑜恒 张洁[1] 李世朋[1] LI Jun;LI Li;LI Yonggan;ZHAO Yanbing;ZHANG Wei;SU Yuheng;ZHANG Jie;LI Shipeng(The Second Department of General Surgery,Jiaozuo People’s Hospital,Jiaozuo,Henan 454002,China)

机构地区：[1]焦作市人民医院普外二区,河南焦作454002

出　　处：《中国普通外科杂志》2021年第1期64-70,共7页China Journal of General Surgery

基　　金：河南省重点研发与推广专项(科技攻关)基金资助项目(202102310126);河南省医学科技攻关计划联合共建资助项目(LHGJ20191342)。

摘　　要：背景与目的:长链非编码RNA HULC(lncRNA HULC)在肝癌中特异性高表达,是肝癌重要的肿瘤标志物。本研究探讨血清lncRNA HULC水平与乙型肝炎病毒相关性肝细胞癌(乙肝相关性肝癌)患者临床特征及预后的关系。方法:收集2012年6月—2017年9月30例接受手术治疗的乙肝相关性肝癌患者血清与手术标本。用qRT-PCR检测患者血清lncRNA HULC的含量,免疫组织化学法检测患者癌组织中肿瘤侵袭转移相关标志物VEGF、MMP-2、E-cadherin的表达。分析患者血清lncRNA HULC水平与患者临床病理因素、侵袭转移相关标志物表达及术后预后的关系。结果:患者血清lncRNA HULC相对水平范围为2.6~9.5,以中位值5.0为界,将患者分为高lncRNA HULC组(12例)与低lncRNA HULC组(18例)。统计分析结果显示,低lncRNA HULC组病理高分化比例高于高HULC组;高lncRNA HULC组III~IV期比例高于低lncRNA HULC组;HULC-L组肝内转移与远处转移例数均比例低于HULC-H组肝癌患者;高lncRNA HULC组肝癌切除术后复发比例高于低lncRNA HULC组肝癌患者,差异均有统计学意义(均P<0.05)。免疫组化结果显示,与低lncRNA HULC组比较,高lncRNA HULC组患者肝癌组织中促肿瘤侵袭转移蛋白VEGF、MMP-2的阳性表达增加,而抑制促肿瘤侵袭转移蛋白E-cadherin的阳性表达明显减少。Kaplan-Meier法分析结果显示,高lncRNA HULC组患者生存率低于低lncRNA HULC组患者,且肝癌切除术后复发率高于低lncRNA HULC组肝癌患者(均P<0.05)。Cox比例风险回归分析结果显示,术前血清HULC水平是乙肝相关性肝癌患者预后的独立影响因素(OR=1.769,P=0.045)。结论:血清lncRNA HULC水平与乙肝相关性肝癌患者恶性临床特征密切相关,高血清lncRNA HULC水平的乙肝相关性肝癌患者预后不良。Background and Aims:Long non-coding RNA HULC(lncRNA HULC)is specifically highly expressed in hepatocellular carcinoma(HCC),and is an important tumor marker of HCC.This study was conducted to investigate the relations of serum lncRNA HULC level with the clinical features and prognosis of patients with hepatitis B virus-related HCC(HBV-related HCC).Methods:The samples of serum and surgical specimens of 30 patients with HBV-related HCC undergoing surgical treatment from June 2012 to September 2017 were collected.The relative levels of lncRNA HULC in the serum of the patients were determined by qRT-PCR,and the expressions of tumor invasion/metastasis-associated markers VEGF,MMP-2 and E-cadherin in the cancer tissues of the patients were detected by immunohistochemical staining.The relations of serum lncRNA HULC level with the clinicopathologic factors and expressions of tumor invasion/metastasis-associated markers as well as the prognosis of the patients were analyzed.Results:The relative level of serum lncRNA HULC of the patients ranged from 2.6 to 9.5.Using the median value of 5.0 as the threshold,the patients were divided into high lncRNA HULC group(12 cases)and low lncRNA HULC group(18 cases).Results of statistical analysis showed that the proportion of cases with high pathological differentiation in low lncRNA HULC group was significantly higher than that in high lncRNA HULC group;the proportion of stage III-IV cases in high lncRNA HULC group was significantly higher than that in low lncRNA HULC group;the proportion of cases with intrahepatic and distant metastasis in low lncRNA HULC group was significantly lower than that in high lncRNA HULC group;the proportion of cases with recurrence in high lncRNA HULC group was significantly higher than that in low lncRNA HULC group,and all the differences had statistical significance(all P<0.05).Results of immunohistochemical staining showed that the positive expressions of tumor invasion/metastasis-promoting proteins VEGF and MMP-2 were increased while the tumor invasion

关 键 词：癌 肝细胞 乙型肝炎病毒 肿瘤侵润 预后 

分 类 号：R735.7[医药卫生—肿瘤]