Transcriptome analysis of molecular mechanisms underlying facial nerve injury repair in rats  

作　　者：Qian-Qian Cao Shuo Li Yan Lu Di Wu Wei Feng Yong Shi Lu-Ping Zhang 

机构地区：[1]Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education,Co-innovation Center of Neuroregeneration,Nantong University,Nantong,Jiangsu Province,China [2]Department of Otolaryngology,Affiliated Hospital of Nantong University,Nantong,Jiangsu Province,China [3]Department of Otolaryngology,Head and Neck Surgery,Eye,Ear,Nose and Throat Hospital,Fudan University,Shanghai,China

出　　处：《Neural Regeneration Research》2021年第11期2316-2323,共8页中国神经再生研究（英文版）

摘　　要：Although the transcriptional alterations inside the facial nucleus after facial nerve injury have been well studied,the gene expression changes in the facial nerve trunk after injury are still unknown.In this study,we established an adult rat model of facial nerve crush injury by compressing the right lateral extracranial nerve trunk.Transcriptome sequencing,differential gene expression analysis,and cluster analysis of the injured facial nerve trunk were performed,and 39 intersecting genes with significant variance in expression were identified.Gene Ontology annotation and Kyoto Encyclopedia of Genes and Genomes pathway analyses of the 39 intersecting genes revealed that these genes are mostly involved in leukocyte cell-cell adhesion and phagocytosis and have essential roles in regulating nerve repair.Quantitative real-time polymerase chain reaction assays were used to validate the expression of pivotal genes.Finally,nine pivotal genes that contribute to facial nerve recovery were identified,including Arhgap30,Akr1b8,C5ar1,Csf2ra,Dock2,Hcls1,Inpp5d,Sla,and Spi1.Primary Schwann cells were isolated from the sciatic nerve of neonatal rats.After knocking down Akr1b8 in Schwann cells with an Akr1b8-specific small interfering RNA plasmid,expression levels of monocyte chemoattractant protein-1 and interleukin-6 were decreased,while cell proliferation and migration were not obviously altered.These findings suggest that Akr1b8 likely regulates the interaction between Schwann cells and macrophages through regulation of cytokine expression to promote facial nerve regeneration.This study is the first to reveal a transcriptome change in the facial nerve trunk after facial nerve injury,thereby revealing the potential mechanism underlying repair of facial nerve injury.This study was approved by the Animal Ethics Committee of Nantong University,China in 2018(approval No.S20180923-007).

关 键 词：Akr1b8 cell proliferation facial nerve injury Gene-Act Networks inflammatory response RNA-SEQ Schwann cells transcriptomics analysis 

分 类 号：R446[医药卫生—诊断学] R745.12[医药卫生—临床医学]