RYBP通过激活PARP-1诱导Parthanatos增加食管鳞癌细胞对YM155的敏感性  

RYBP activates PARP-1 induced Parthanatos in esophageal squamous cell carcinoma cells and enhances response to YM155

作　　者：柯悦 李宇星 师小博 郭伟[1] 刘笑笑 王玉琛 阮琴利 潘纪元 杨晓平[2] KE Yue;LI Yuxing;SHI Xiaobo;GUO Wei;LIU Xiaoxiao;WANG Yuchen;RUAN Qinli;Pan Jiyuan;YANG Xiaoping(Department of Radiotherapy,The Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710004,China;Department of Thoracic Surgery,The Second Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710004,China)

机构地区：[1]西安交通大学第二附属医院肿瘤放疗科,陕西西安710004 [2]西安交通大学第二附属医院胸外科,陕西西安710004

出　　处：《西安交通大学学报（医学版）》2021年第2期233-237,256,共6页Journal of Xi’an Jiaotong University（Medical Sciences）

摘　　要：目的研究RYBP通过激活PARP-1依赖的Parthanatos增加食管鳞癌细胞对YM155敏感性的作用。方法使用CCK-8实验和流式细胞实验分析YM155对过表达RYBP和对照组细胞的抑制率及细胞死亡百分比,使用Western blotting分析YM155处理细胞后Parthanatos相关蛋白质表达的变化。结果通过YM155处理后,过表达RYBP组的细胞抑制率及细胞死亡百分比明显高于对照组;过表达RYBP能明显激活PARP-1,并在YM155处理后,过表达组的PARP-1活化更为显著;YM155处理后,细胞核内PAR积累,AIF由线粒体进入细胞核,且在RYBP过表达组的上述效应更为显著。结论 RYBP能通过激活PARP-1/PAR/AIF介导的Parthanatos增加食管鳞癌细胞对YM155的敏感性。Objective To explore the role of RYBP in activating PARP-1 dependent Parthanatos and promoting response to YM155 in esophageal squamous cell carcinoma. Methods CCK-8 and flow cytometry were used to analyze the inhibition ratio and cell death percentage after YM155 treatment in both RYBP overexpression group and control group. Western blotting was used to detect the expression of Parthanatos-related proteins. Results Compared with control group, RYBP overexpression group showed higher inhibition ratio and cell death percentage after YM155 treatment. Overexpression of RYBP activated PARP-1 with or without YM155 treatment. Besides, after YM155 treatment, KYSE170-RYBP showed more PAR accumulation in the nucleus, AIF translocation from mitochondria to the nucleus than control cells. Conclusion RYBP can activate PARP-1/PAR/AIF-dependent induced Parthanatos in esophageal squamous cell carcinoma and enhance response to YM155.

关 键 词：食管鳞癌 RYBP PARP-1 YM155 Parthanatos 

分 类 号：R735.1[医药卫生—肿瘤] R815[医药卫生—临床医学]