不同TiO2纳米管表面对成骨细胞黏附的研究  

Adhesion and growth of osteoblasts on TiO nanotubes with different diameters and lengths

作　　者：徐玲[1] 于卫强[1] XU Ling;YU Wei-qiang(Department of prosthodontics,The Ninth People'hospital,Shanghai Jiaotong University,Shanghai Key Ixiboralory of Stomatology,Shanghai 200011,China.)

机构地区：[1]上海交通大学医学院附属第九人民医院·口腔医学院口腔修复科,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所,上海200011

出　　处：《临床口腔医学杂志》2021年第1期3-7,共5页Journal of Clinical Stomatology

基　　金：国家自然科学基金青年基金项目资助(81700934);上海交通大学医学院附属第九人民医院基础研究助推计划资助(JYZZ107)。

摘　　要：目的:研究MC3T3-E1成骨细胞在不同直径和长度TiO2纳米管上的黏附/生长情况。方法:选取固定长度约500 nm,直径约30 nm/80 nm的TiO2纳米管,以及固定直径约80 nm,厚度约500 nm/1.5μm的TiO2纳米管材料,体外研究其成骨细胞的黏附生长情况。结果:细胞形态和细胞计数显示成骨细胞在直径为30 nm的TiO2纳米管表面的黏附力明显高于其在直径为80 nm的纳米管表面。MTT和细胞计数结果显示,1.5μm厚的纳米管能促进MC3T3-E1成骨细胞的增殖,明显高于较薄的纳米管层(P<0.05)。结论:成骨细胞的黏附和形态主要受纳米管直径的影响,而细胞的增殖主要受纳米管长度的影响。Objective:This experiment investigated the adhesion/growth of MC3 T3-E1 osteoblasts on TiO2 nanotubes with different diameters and lengths.Methods:TiO2 nanotubes with a fixed length of about 500 nm,a diameter of about 30 nm or 80 nm and a fixed diameter of about 80 nm TiO2 nanotubes with a thickness of about 500 nm or 1.5 μm were used to study the adhesion growth of osteoblasts in vitro.Results:Cell morphology and the cell count showed that the adhesion of osteoblasts on the surface of TiO2 nanotubes with a diameter of 30 nm was significantly higher than that on the surface of nanotubes with a diameter of 80 nm.MTT and cell count results showed that 1.5 μm thick nanotubes could promote the proliferation of MC3 T3-E1 osteoblasts,which was significantly higher than the thinner nanotube layer(P<0.05).Conclusion:The adhesion and morphology of osteoblasts are mainly affected by nanotube diameter,while cell proliferation is mainly affected by nanotube length.

关 键 词：TIO2纳米管 成骨细胞 细胞黏附 细胞增殖 

分 类 号：R783.1[医药卫生—口腔医学]