姜藤茶对寒凝血瘀痛经大鼠的影响  

Effect of Jiangteng Tea on the Cold Coagulation Stasis Dysmenorrhea in Rats

作　　者：孟君 秦画置 周扬 李丹 刘昊奇 邓郊梅 杨知 MENG Jun;QIN Hua-zhi;ZHOU Yang;LI Dan;LIU Hao-qi;DENG Jiao-Mei;YANG Zhi(Zhuzhou Qianjin Pharmaceutical Co.,Ltd.,Zhuzhou,Hunan 412000,China;Hunan slakejingda experimental animal Co.,Ltd.,Changsha,Hunan 400125,China)

机构地区：[1]株洲千金药业股份有限公司,株洲412000 [2]湖南斯莱克景达实验动物有限公司,长沙400125

出　　处：《粮油食品科技》2021年第2期186-192,共7页Science and Technology of Cereals,Oils and Foods

摘　　要：姜藤茶由红糖、姜、藤茶、低聚木糖和菊花等组成,观察姜藤茶对寒凝血瘀痛经的药效学试验,为临床试验提供参考资料。实验注射苯甲酸雌二醇制备痛经模型,同步灌胃给药姜藤茶10 d,最后一次给药后1 h,腹腔注射缩宫素10 U/只,观察30 min内大鼠疼痛反应,以扭体反应为子宫强烈收缩(痛经)的指标,测定β-内腓肽(β-EP)和6酮前列腺素F1α的浓度,并取子宫、卵巢,HE染色进行组织学检查。与模型组比较,姜藤茶低、中、高剂量组、痛经宝组和阿司匹林组大鼠扭体次数显著降低(P<0.01);姜藤茶高剂量组和痛经宝组大鼠β-EP显著升高(P<0.01)。结果表明姜藤茶能减轻痛经大鼠的扭体次数,提高了β-内腓肽和6酮前列腺素F1α水平含量,对大鼠痛经有较好改善作用。同时,用冰浴+盐酸肾上腺素制备寒凝血瘀模型,同步灌胃给药姜藤茶7 d,检测血液流变学指标及红细胞压积。与模型组比较,药物组红细胞压积均有减少,但都未见明显差异(P>0.05),姜藤茶中剂量组全血低切粘度和低切相对值有明显降低(P<0.05);姜藤茶高剂量组全血低、中、高切粘度,低切相对值和红细胞聚集指数显著降低(P<0.01),卡松粘度明显降低(P<0.05)。结果表明姜藤茶能降低寒凝血瘀模型大鼠全血粘度及血浆粘度,提示姜藤茶对寒凝血瘀证有一定的改善。这些研究表明姜藤茶对痛经和寒凝血瘀,有一定疗效,具有舒缓痛经等功能。Jiangteng tea is composed of brown sugar,ginger,Teng tea,xylooligosaccharide and chrysanthemum.To observe the pharmacodynamic test of Jiangteng tea on cold blood stasis dysmenorrhea,and provide reference materials for the clinical trials,a dysmenorrhea model was prepared by injecting estradiol benzoate,and ginger vine tea was administered simultaneously by intragastric administration for 10 days.One hour after the last administration,10 U of oxytocin was injected intraperitoneally.And the rat's pain response was observed within 30 minutes.The writhing response was selected as an indicator of strong uterine contraction(dysmenorrhea),the concentration ofβ-endorphin(β-EP)and 6-ketoprostaglandin F1αwere measured,at the same time,the uterus and ovaries were taken and HE stained for histological examination.The results showed that Jiangteng tea can reduce the number of writhing in rats with dysmenorrhea,increase the levels ofβ-endophorin and 6-ketoprostaglandin F1α,and have a better effect on improving dysmenorrhea.At the same time,an ice bath+epinephrine hydrochloride was used to prepare a cold coagulation blood stasis model,and Jiangteng tea was administered by gavage simultaneously for 7 days with hemorheological indexes and hematocrit were measured.Compared with the model group,the hematocrit in the drug group was reduced,but there was no significant difference(P>0.05).The low-shear viscosity and low-shear relative value of whole blood in the Jiangtengcha medium-dose group were significantly reduced(P<0.05).In the high-dose Jiangteng tea group,whole blood low,medium and high shear viscosity,low shear relative value and erythrocyte aggregation index were significantly reduced(P<0.01),and Kasson viscosity was significantly reduced(P<0.05).The results showed that Jiangteng tea can reduce the whole blood viscosity and plasma viscosity of rats with cold coagulation and blood stasis,suggesting that Jiangteng tea has certain improvement on cold coagulation and blood stasis syndrome.These studies showed that Jiangteng

关 键 词：姜藤茶 痛经 寒凝血瘀 Β-内腓肽 6酮前列腺素F1α 

分 类 号：TS272.5[农业科学—茶叶生产加工]