浑圆链霉菌QH−16鉴定及抑菌活性产物的结构研究  

Identification of Actinomycete Strain QH-16 and Structural Analysis of Its Active Products

作　　者：李志田 蔡淑琳 王合 杨海青 赵娟 刘德文[2] 刘娅[1] 张殿朋[2] LI Zhitian;CAI Shulin;WANG He;YANG Haiqing;ZHAO Juan;LIU Dewen;LIU Ya;ZHANG Dianpeng(School of Food,Shihezi University,Shihezi 832000,China;Institute of Plant and Environment Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;Forest Pest Management and Quarantine Station of Beijing,Beijing 100029,China;Beijing Pinggu District Fruit Office,Beijing 101200,China)

机构地区：[1]石河子大学食品学院,石河子832003 [2]北京市农林科学院植物保护环境保护研究所,北京100097 [3]北京市林业保护站,北京100029 [4]北京市平谷区果品办公室,北京101200

出　　处：《中国生物防治学报》2021年第1期156-164,共9页Chinese Journal of Biological Control

基　　金：北京市农林科学院科技创新能力建设专项(KJCX20200203,KJCX20180408)。

摘　　要：拮抗放线菌能够产生具有较高商业价值的生物活性物质,应用潜力巨大。本研究以桃细菌性穿孔病菌Xanthomonas arboricola pv.pruni为指示菌,采用平板对峙法从已有拮抗菌库中筛选对病原菌具有抑制作用的拮抗放线菌,发现菌株QH−16的拮抗效果最佳,通过16S rDNA序列分析,结合形态特征、培养特性和生理特性,鉴定该菌为浑圆链霉菌Streptomyces globosus。离体测定其对多种植物病原菌及细菌具有很好的抑菌作用,琼脂平板打孔法测定对禾谷镰刀菌的抑菌带宽达14 mm;对枯草芽胞杆菌抑菌圈直径达24 mm。采用溶剂萃取法提取菌株QH−16发酵产物的活性成分,通过硅胶柱层析、制备型薄层色谱(PLC)以及循环制备型色谱制备纯品化合物,经核磁共振法解析,确定其中的抑菌活性单体化合物为邻苯二甲酸二丁酯。Antagonistic actinomycetes can produce bioactive compounds with high commercial value and great application potential.In this study,Xanthomonas arboricola pv.pruni was used as the indicator bacteria,and five antagonistic actinomycetes showing inhibition action on pathogen by dual culture assay were screened and strain QH-16 presented the highest antagonistic efficacy.Strain QH-16 was identified by 16S rDNA sequence analysis,morphological observation,cultural and physiological characteristics.The results showed that strain QH-16 was identified as Streptomyces globosus.The filtrate from ferment broth of strain QH-16 could inhibit Fusarium graminearum and Bacillus subtilis,with inhibition bands of 14 mm and 22 mm,respectively.The active ingredients of the fermentation products were extracted by solvent extraction and purified by silica gel column chromatography,thin layer chromatography for preparation(PLC)and preparative analyzed by nuclear magnetic resonanc,the main active product in the fermentation products was identified to be dibutyl phthalate.

关 键 词：桃细菌性穿孔病菌 放线菌 鉴定 活性物质 

分 类 号：S467[农业科学—植物保护]