不同类型Zn胁迫对1株好氧反硝化细菌功能影响的比较研究  

Toxicity Effects of Different Types of Zn Stress on a Strain of Aerobic Denitrifying Bacteria Compare Research

作　　者：吴春晗 白洁[1,2] 赵阳国[1,2] 任朝萌 陈琳 孙鹏飞[1] 李岿然[3] WU Chun-Han;BAI Jie;ZHAO Yang-Guo;REN Zhao-Meng;CHEN Lin;SUN Peng-Fei;LI Kui-Ran(College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China;The Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China;College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China)

机构地区：[1]中国海洋大学环境科学与工程学院,山东青岛266100 [2]中国海洋大学海洋环境与生态教育部重点实验室,山东青岛266100 [3]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《中国海洋大学学报（自然科学版）》2021年第5期96-104,共9页Periodical of Ocean University of China

基　　金：国家重点研究发展计划项目(2018YFC1407601)资助。

摘　　要：本文通过分析在不同浓度普通氧化锌(ZnO)、硫酸锌(ZnSO4)及纳米氧化锌(ZnO-NPs)胁迫下1株筛自胶州湾的好氧反硝化菌Zobellella sp.B307生长量和反硝化能力的变化,比较研究了不同类型Zn对该菌株生长及脱氮功能的影响。结果表明,ZnO-NPs对该菌株的生长有明显的持续抑制作用,显著降低其最大生长量,并对其脱氮功能产生持续抑制;ZnSO_(4)对菌株生长及脱氮功能前期抑制作用较强,后期抑制效应逐渐减弱;普通ZnO对菌株生长及脱氮功能并不产生显著影响。3种类型锌对该菌株的功能抑制表现为ZnO-NPs抑制作用最强,其次是ZnSO_(4),普通ZnO的抑制相对较弱,产生影响差异的主要原因与这三种类型Zn化合物作用机制的不同有关。研究结果可为深入探讨不同类型的Zn污染对近海沉积物氮素循环产生的潜在影响提供依据。To study the effects of different types of Zn on the growth and denitrification of an aerobic denitrifying bacterium Zobellella sp.B307 isolated from Jiaozhou Bay,the changes of strain growth and denitrification capacity under different concentrations of common zinc oxide(ZnO),zinc sulfate(ZnSO_(4))and nano-zinc oxide(ZnO-NPs)were compared in this article.The results showed that ZnO-NPs had a significant sustained inhibitory effect on the growth of this strain,significantly reduced its maximum growth amount,and sustainedly inhibited its denitrification function.ZnSO_(4) inhibited the growth and denitrification of the strain in the early stage,while the inhibitory effect was gradually weakened in the later stage.Ordinary ZnO has no significant effect on the strain.The functional inhibition of the three types of zinc on this strain showed that ZnO-NPs had the strongest inhibitory effect,followed by ZnSO4,and ordinary ZnO showed relatively weak inhibition,which is mainly related to the different mechanisms of action of these three types of Zn.The results of the study can provide a basis for further exploring the potential impact of different types of Zn pollution on the nitrogen cycle of offshore sediment.

关 键 词：锌 纳米氧化锌 好氧反硝化细菌 OD600 

分 类 号：X171[环境科学与工程—环境科学]