肝细胞生长因子/酪氨酸蛋白激酶MET 信号通路与乳腺癌侵袭转移的相关性分析  

Analysis of correlation between HGF/c-met signaling pathway and invasion and metastasis of breast cancer

作　　者：刘海旺[1] 张宏旭[2] 刘燃[3] 郝美玲[1] 王军[1] 李春辉[1] LIU Haiwang;ZHANG Hongxu;LIU Ran;HAO Meiling;WANG Jun;LI Chunhui(Department of Pathology,Affiliated Hospital of Chengde Medical College,Chengde,Hebei 067000,China;Breast Surgery,Affiliated Hospital of Chengde Medical College,Chengde,Hebei 067000,China;Anesthesiology,Affiliated Hospital of Chengde Medical College,Chengde,Hebei 067000,China)

机构地区：[1]承德医学院附属医院病理科,河北承德067000 [2]承德医学院附属医院乳腺外科,河北承德067000 [3]承德医学院附属医院麻醉科,河北承德067000

出　　处：《安徽医药》2021年第3期516-519,共4页Anhui Medical and Pharmaceutical Journal

基　　金：承德市科学技术研究与发展项目(201701A080)

摘　　要：目的探讨分析肝细胞生长因子(HGF)/酪氨酸蛋白激酶MET(c-met)信号通路与乳腺癌侵袭转移的相关性。方法选择2018年3月至2019年3月承德医学院附属医院临床术后经病理证实的乳腺癌标本75例、乳腺纤维腺瘤标本60例以及癌旁正常乳腺组织标本50例。采用SP免疫组化法以及逆转录聚合酶链反应(RT-PCR)法检测各组织标本HGF、c-met、β连环素(β-catenin)蛋白表达和mRNA表达情况。结果HGF[82.67%(62/75)比48.33%(29/60)比14.00%(7/50)]、c-met[86.67%(65/75)比55.00%(33/60)比20.00%(10/50)]、β-catenin[78.67%(59/75)比41.67%(25/60)比6.00%(3/50)]在乳腺癌组织、乳腺纤维腺瘤组织以及癌旁正常乳腺组织中的表达均差异有统计学意义(P<0.05)。在乳腺癌、乳腺纤维腺瘤以及癌旁正常乳腺组织中HGF、c-met mRNA相对表达量差异有统计学意义(P<0.05),其中乳腺癌相对表达量最高、乳腺纤维腺瘤相对表达量次之;而乳腺癌、乳腺纤维腺瘤以及癌旁正常乳腺组织中β-catenin mRNA相对表达量比较差异无统计学意义(P>0.05)。HGF表达阳性率与病人TNM分期、淋巴结转移、肿瘤大小、ER表达、HER-2表达有关(P<0.05)。c-met蛋白表达阳性率与病人肿瘤TNM分期、淋巴结转移密切相关(P<0.05)。β-catenin蛋白表达异常率与病人TNM分期、淋巴结转移、肿瘤大小密切相关(P<0.05)。结论乳腺癌组织中HGF、c-met蛋白高表达而β-catenin蛋白异常表达,且HGF/c-met信号通路及β-catenin蛋白与乳腺癌侵袭转移密切相关。Objective To investigate the correlation between HGF/c-met signaling pathway and invasion and metastasis of breast cancer.Methods From March 2018 to March 2019,75 breast cancer specimens,60 breast fibroadenoma specimens and 50 normal breast tissue specimens were selected from the Affiliated Hospital of Chengde Medical College.The expression of HGF,c-met andβ-catenin protein and the expression of HGF,c-met andβ-catenin mRNA were detected by SP immunohistochemistry and RT-PCR.Re⁃sults There were significant differences in the expression of HGF[82.67%(62/75)vs.48.33%(29/60)vs.14.00%(7/50)],c-met[86.67%(65/75)vs.55.00%(33/60)vs.20.00%(10/50)]andβ-catenin[78.67%(59/75)vs.41.67%(25/60)vs.6.00%(3/50)]in breast cancer,breast fibroadenoma and adjacent normal breast tissues(P<0.05).The relative expression of HGF and c-Met mRNA in breast cancer,breast fi⁃broadenoma and adjacent normal breast tissues were significantly different(P<0.05),and the relative expression of HGF and c-Met mRNA in breast cancer was the highest,followed by that in breast fibroadenoma.There was no significant difference in the relative ex⁃pression ofβ-catenin protein between breast cancer,breast fibroadenoma and adjacent normal breast tissues(P>0.05).The positive rate of HGF expression was related to TNM stage,lymph node metastasis,tumor size,ER expression and HER-2 expression(P<0.05).The positive rate of c-met protein expression was closely related to TNM stage and lymph node metastasis(P<0.05).The abnormal ex⁃pression rate ofβ–catenin protein was closely related to TNM stage,lymph node metastasis and tumor size(P<0.05).Conclusions The expression of HGF and c-Met protein in breast cancer tissues is up-regulated while that ofβ-catenin protein is abnormal.HGF/cmet signaling pathway andβ-catenin protein are closely related to the invasion and metastasis of breast cancer.

关 键 词：乳腺肿瘤 肝细胞生长因子 受体蛋白质酪氨酸激酶类 Β连环素 HGF/c-met信号通路 侵袭 转移 相关性 

分 类 号：R737.9[医药卫生—肿瘤]