黄柏胶囊联合盐酸奥洛他定对急性湿疹模型大鼠的干预作用及抗过敏机制探讨  

Intervention effect of Huangbai capsule combined with olotatidine hydrochloride on acute eczema model rats and its anti-allergic mechanism

作　　者：戴雅琴 杜崇民 谢惠平 DAI Yaqin;DU Chongmin;XIE Huiping(The Third People’s Hospital of Hubei Province Affiliated to Jianghan University, Wuhan 430000, Huebei, China;Jimo District Hospital of Traditional Chinese Medicine, Qingdao 266200, Shandong, China)

机构地区：[1]江汉大学附属湖北省第三人民医院,湖北武汉430033 [2]青岛市即墨区中医医院,山东青岛266200

出　　处：《现代中西医结合杂志》2021年第9期926-930,1005,共6页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：湖北省自然科学基金资助项目(2018CFB117)。

摘　　要：目的探讨黄柏胶囊联合盐酸奥洛他定对急性湿疹模型大鼠的干预作用及抗过敏机制。方法将50只SD大鼠随机分为对照组、模型组、黄柏胶囊组、盐酸奥洛他定组和联合组,每组10只。除对照组外,其余组采用右侧背部涂抹2,4-二硝基氯苯(DNCB)方法建立急性湿疹大鼠模型。于造模后第10天,对照组和模型组给予生理盐水灌胃,黄柏胶囊组给予黄柏胶囊灌胃,盐酸奥洛他定组给予盐酸奥洛他定灌胃,联合组给予黄柏胶囊和盐酸奥洛他定灌胃,均1次/d,连续10 d。各组于造模后第20天进行湿疹度评分、皮肤肿胀度评价、HE染色组织病理学观察,ELISA法检测血清炎症因子干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)和肿瘤坏死因子-α(TNF-α)水平,分别采用Western blot和RT-PCR法检测皮肤组织中细胞间黏附分子-1(ICAM-1)和血管内皮细胞黏附分子-1(VCAM-1)蛋白及mRNA表达量。结果黄柏胶囊组、盐酸奥洛他定组和联合组的湿疹度评分、皮肤肿胀度及血清IFN-γ、IL-4、TNF-α水平均显著低于模型组(P均<0.05),病理形态显著改善,且联合组各指标均显著低于黄柏胶囊组和盐酸奥洛他定组(P均<0.05);黄柏胶囊组、盐酸奥洛他定组和联合组皮肤组织中ICAM-1和VCAM-1蛋白及mRNA表达量均显著低于模型组(P均<0.05),且联合组均显著低于黄柏胶囊组和盐酸奥洛他定组(P均<0.05)。结论黄柏胶囊联合盐酸奥洛他定治疗可明显减轻急性湿疹大鼠皮损,改善组织病理形态,可能与下调炎症因子和ICAM-1、VCAM-1蛋白及mRNA表达有关。Objective It is to investigate the intervention effect of Huangbai capsule combined with olotatidine hydrochloride on acute eczema model rats and its anti-allergic mechanism.Methods Fifty SD rats were randomly divided into control group,model group,Huangbai capsule group,olotatidine hydrochloride group and combination group,each group had 10 rats.Except for the control group,the acute eczema rats models were established by applying 2,4-dinitrochlorobenzene(DNCB)on the right back.On the 10th day after model establishment,the control group and model group were given 0.9%saline by gavage,the Huangbai capsule group was given Huangbai capsule by gavage,the olotatidine hydrochloride group was given olotatidine hydrochloride by gavage,and the combination group was given Huangbai capsule and olotatidine hydrochloride by gavage,all one time per day,continuously treated for 10 days.The score of eczema,skin swelling were evaluated and the He staining histopathological changes were observed on the 20th day after model establishment.The levels of serum inflammatory factor interferon-gamma(IFN-γ),interleukin-4(IL-4)and tumor necrosis factor-alpha(TNF-α)were detected by ELISA.The protein and mRNA expression levels of intercellular adhesion molecule-1(ICAM-1)and vascular endothelial cell adhesion molecule-1(VCAM-1)were detected by Western blot and RT-PCR.Results The score of eczema and skin swelling and the levels of serum IFN-γ,IL-4 and TNF-αin Huangbai capsule group,olotatidine hydrochloride group and combination group were significantly lower and the pathological morphology were significantly improved compared with model group,the scores and levels in the combination group were lower than those in Huangbai capsule group and olotatidine hydrochloride group(P<0.05).The protein and mRNA expression levels of ICAM-1 and VCAM-1 in the Huangbai capsule group,olotatidine hydrochloride group and combination group were significantly lower than those in the model group(P<0.05),and the combination group was significantly lower than t

关 键 词：急性湿疹 黄柏胶囊 盐酸奥洛他定 炎症反应 细胞间黏附分子-1 血管内皮细胞黏附分子-1 

分 类 号：R-332[医药卫生]