Elevated miR-10a-5p facilitates cell cycle and restrains adipogenic differentiation via targeting Map2k6 and Fasn,respectively  

作　　者：Xiaoyu Wang Huifang Zhang Meixue Xu Xin'E Shi Gongshe Yang Shiduo Sun Xiao Li 

机构地区：[1]Key Laboratory of Animal Genetics,Breeding and Reproduction of Shaanxi Province,College of Animal Sciences and Technologies,Northwest A&F University,Yangling 712100 China

出　　处：《Acta Biochimica et Biophysica Sinica》2020年第11期1227-1235,共9页生物化学与生物物理学报（英文版）

基　　金：the grant from the National Science and Technology Major Project of China(No.2016ZX08006003).

摘　　要：miRNAs are a small class of noncoding RNAs that perform biological functions by regulating the stability or translation of target genes in various biological processes.This study illustrated the role of miR-10a-5p,which is relatively enriched in adipose tissues,using primary mouse preadipocytes as model.With elevated miR-10a-5p expression,the proliferative ability of mouse preadipocytes was significantly enhanced,indicated by increased EdU+cells and G1/S transition,accompanied by upregulated Cyclin B,Cyclin D and PCNA and downregulated p21 and p27.Meanwhile,the adi-pogenic differentiation was significantly attenuated by elevated miR-10a-5p,supported by Oil Red 0 staining and suppressed PPARγand aP2 expression.Furthermore,Map2k6 and Fasn were pre-dicted to be the target genes of miR-10a-5p in silico,and dual luciferase reporter assay confirmed the direct targeting effects.Western blot analysis results showed that miR-10a-5p specially reduced Map2k6 expression at the proliferative stage without affecting Fasn expression,while significantly restrained Fasn expression with unchanged Map2k6 expression during adipogenic differentiation.Taken together,these results revealed a potential role of miR-10a-5p in adipogenesis and in the treatment of obesity.

关 键 词：MIRNA cell cycle ADIPOGENESIS miR-10a-5p PREADIPOCYTES 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]