辽宁省HIV-1新发重组毒株近全长基因组特征分析  

Near full-length genomic characteristics of unique recombinant HIV-1 in Liaoning province

作　　者：赵砚[1] 吕娅妮[1] 康续 辛若雷[2] 鄂爽[1] 王莉[1] ZHAO Yan;LYU Ya-ni;KANG Xu;XIN Ruo-lei;E Shuang;WANG Li(Liaoning Provincial Center for Disease Control and Prevention,Shenyang,Liaoning 110005,China;不详)

机构地区：[1]辽宁省疾病预防控制中心艾滋病与性传播疾病预防控制所,辽宁沈阳110005 [2]北京市疾病预防控制中心

出　　处：《中国国境卫生检疫杂志》2021年第1期1-6,共6页Chinese Journal of Frontier Health and Quarantine

基　　金：国家自然科学基金项目(81273136)。

摘　　要：目的对辽宁省HIV-1耐药检测中发现的1份未明确分型的样本进行近全长基因组序列分析,阐明其重组模式及可能的亲本来源。方法提取病毒RNA并逆转录为cDNA,用近末端稀释法分两段对HIV-1近全长基因组进行扩增,对稀释度高的阳性扩增孔进行Sanger法测序。用SimPlot 3.5.1软件确定重组模式,并按重组断点分区域构建邻接法系统进化树,确定亲本毒株的可能来源。结果经扩增和测序获得8957核苷酸的HIV-1近全长基因组序列。在CRF01AE骨架上,其gag基因全长和pol基因大部分片段被B亚型毒株相应片段重组替换,共有6个基因片段。亲本来源分析表明,该基因组片段Ⅰ(HXB2 nt 641-2901)、Ⅲ(3514-3845)、Ⅴ(4026-4836)来自于B亚型毒株(HQ215554),片段Ⅱ(HXB2 nt 2902-3513)、Ⅳ(3846-4025)、Ⅵ(4837-9606)来自于g4a CRF01AE毒株(CYM059),均来自北方男男性行为人群。在检测到M184V、T215F和K103N、N348I耐药突变后更换克力芝为主的二线治疗方案,获得较好的病毒抑制效果。结论在辽宁省发现1株HIV-1新发重组毒株,其亲本来自我国北方男男性行为人群中流行的g4a CRF01AE与B亚型毒株。Objective To investigate the genetic characteristics and the possible source for parent strains,the near fulllength genomic sequence of a novel recombinant strain detected during the surveillance of HIV-1 drug resistance in Liaoning province was obtained.Methods The viral RNA was extracted and reversely transcribed to cDNA.Two overlapping segments of HIV-1 genome were amplified by near-endpoint dilution method,and the positive replicons at high dilutions were sequenced using Sanger method.Recombinant breakpoints were determined using SimPlot 3.5.1 software,and segmental phylogenetic trees were constructed to determine the possible sources for the possible parent strains.Results An 8957 nucleotide of genome sequence HIV-1 was obtained by amplification and sequencing.In the CRF01AE backbone,the whole gag gene and mostly pol gene were recombined and replaced by corresponding segments of subtype B strain,and the genome was genetically divided into six fragments.The fragment Ⅰ(HXB2 nt641-2901),fragment Ⅲ(3514-3845)and fragment Ⅴ(4026-4836)were originated from subtype B strain(HQ215554)and fragment Ⅱ(HXB2 nt 2902-3513),Ⅳ(3846-4025)and Ⅵ(4837-9606)from g4 a CRF01AE strain(CYM059),prevalent in the MSM across northern China.The treatment program was changed to the 2 nd-line regimen of LPV/r upon the observation of drug resistance mutations of M184 V,T215 F and K103 N,N348 I,with good viral suppression.Conclusion A novel recombinant strain was found in Liaoning,and the parent strains were g4 a CRF01AE and B prevalent across northern China.

关 键 词：人类免疫缺陷病毒 重组 系统进化 

分 类 号：R183[医药卫生—流行病学]