萎胃通调汤对大鼠慢性萎缩性胃炎癌前病变及IKKβ,Bcl-2表达的影响  

Effect of Weiwei Tongtiao Decoction on Expression of IKKβ,Bcl-2 in CAG Precancerous Lesion Rats

作　　者：游绍伟 易旭 赵琦 王文素 杨玲 童平珍 王学勇[3] 詹亚梅[4] YOU Shao-wei;YI Xu;ZHAO Qi;WANG Wen-su;YANG Ling;TONG Ping-zhen;WANG Xue-yong;ZHAN Ya-mei(The 2nd Hospital Affiliated Guizhou University of Traditional Chinese Medicine,Guiyang 550003,China;People's Hospital of Changshou Chongqing,Chongqing 401220,China;Beijing University of Chinese Medicine,Beijing 102488,China;Guizhou Provincial People's Hospital,Guiyang 550002,China)

机构地区：[1]贵州中医药大学第二附属医院,贵阳550003 [2]重庆长寿区人民医院,重庆401220 [3]北京中医药大学,北京102488 [4]贵州省人民医院,贵阳550002

出　　处：《中国实验方剂学杂志》2021年第6期55-61,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：贵州省科技计划项目(黔科合基础[2016]1014);贵州省科技合作计划项目(黔科合LH字[2015]7268号);贵州省中医药管理局中医药、民族医药科学技术研究课题项目(QZyy-2014-046);黔科合支撑项目(20172546)。

摘　　要：目的:探讨萎胃通调汤对慢性萎缩性胃炎(CAG)癌前病变大鼠胃黏膜病理及IκB激酶β(IKKβ),B淋巴细胞瘤-2(Bcl-2)表达水平的影响。方法:SD大鼠随机分为正常组、模型组、胃复春组、萎胃通调汤高、中、低剂量组。N-甲基-N’-硝基-N-亚硝基胍(MNNG)复合造模法制备CAG癌前病变大鼠模型。萎胃通调汤高、中、低剂量组、胃复春组分别灌胃给予24,12,6 g·kg^(-1)萎胃通调汤及0.45 g·kg^(-1)胃复春混悬液,1次/d,连续12周。苏木素-伊红(HE)染色观察大鼠胃黏膜组织病理学变化,实时荧光定量聚合酶链式反应(Real-time PCR),免疫组织化学法(IHC)及蛋白免疫印迹法(Western blot)检测大鼠胃黏膜组织IKKβ,Bcl-2 mRNA及蛋白水平表达。结果:与正常组比较,模型组大鼠胃黏膜组织镜下可见100%固有腺体萎缩及轻-重度肠上皮化生,25%低级别上皮内瘤变。胃复春、萎胃通调汤均能改善模型大鼠胃黏膜固有腺体萎缩,中、重度肠上皮化生及低级别上皮内瘤变组织病理损伤,其中高剂量效果明显;与正常组比较,模型组大鼠胃黏膜IKKβ,Bcl-2 mRNA和蛋白表达均上调(P<0.01);与模型组比较,胃复春组与萎胃通调汤高、中、低剂量组大鼠胃黏膜IKKβ,Bcl-2 mRNA和蛋白表达均下调(P<0.05,P<0.01),呈一定的剂量依赖关系,其中萎胃通调汤高剂量组IKKβ,Bcl-2 mRNA和蛋白呈现与正常组相似的表达水平。结论:萎胃通调汤能改善甚至逆转大鼠CAG癌前病变胃黏膜,其干预机制可能与通过下调胃黏膜IKKβ,Bcl-2表达有关。Objective:To investigate the effect of Weiwei Tongtiao decoction on gastric mucosal pathology and the expression level of inhibitor kappa B kinaseβ(IKKβ)and B-cell lymphoma-2(Bcl-2)in rats with chronic atrophic gastritis(CAG)precancerous lesion.Method:SD rats were randomly divided into normal group,model group,positive drug Weifuchun group,Weiwei Tongtiao decoction high,medium and low dose treatment groups.The rat model of CAG precancerous lesion was prepared by N-methyl-N’-nitro-Nnitrosoguanidine(MNNG)compound modeling method.weiwei Tongtiao decoction high,medium and low dose treatment groups received intragastric administration of 24,12,6 g·kg^(-1) Weiwei Tongtiao decoction respectively,while Weifuchun group received 0.45 g·kg^(-1) Weifuchun suspension,once per day for 12 weeks.The pathological changes of gastric mucosa of rats were observed by hematoxylin-eosin(HE)staining,and the mRNA and protein levels of IKKβ and Bcl-2 in gastric mucosa of rats were detected by Real-time quantitative polymerase chain reaction(Real-time PCR),immunohistochemistry(IHC)and Western blot.Result:Compared with the normal group,100%inherent gland atrophy,mild to severe intestinal metaplasia,and 25% low-grade intraepithelial neoplasia were observed under microscope in model group.All Weifuchun group and Weiwei Tongtiao decoction groups could improve the atrophy of gastric glands,moderate to severe intestinal metaplasia and pathological injury of low-grade intraepithelial neoplasia,especially at high dose group.Compared with the normal group,IKKβ,Bcl-2 mRNA and protein expressions in the gastric mucosa of the model group were upregulated(P<0.01).Compared with the model group,the mRNA and protein expressions of IKKβ and Bcl-2 in gastric mucosa of rats in the Weifuchun group and the Weiwei Tongtiao decoction high,medium and low dose groups were down-regulated(P<0.05,P<0.01),showing a dose-dependent relationship,and such levels in the Weiwei Tongtiao decoction high-dose intervention group were similar to those in normal group.Co

关 键 词：萎胃通调汤 慢性萎缩性胃炎 胃癌前病变 IκB激酶β B淋巴细胞瘤-2 

分 类 号：R22[医药卫生—中医基础理论] R242[医药卫生—中医学]