Evodiamine inhibits high-fat diet-induced colitis-associated cancer in mice through regulating the gut microbiota  

作　　者：Li-qing Zhu Li Zhang Jia Zhang Guo-lin Chang Gang Liu Dan-dan Yu Xiao-min Yu Mi-sheng Zhao Bin Ye 

机构地区：[1]Department of Pathogenic Biology,Chongqing Medical University,Chongqing 400016,China Research Center for Molecular Medicine and Tumor,Chongqing Medical University,Chongqing 400016,China Department of Clinical Laboratory,The First Affiliated Hospital of Wenzhou Medical University,Wenzhou 325023,Zhejiang Province,China Department of Pathophysiology,Chongqing Medical University,Chongqing 400016,China Department of Clinical Laboratory,Wenzhou People’s Hospital,Wenzhou 325023,Zhejiang Province,China Department of Emergency,University-Town Hospital of Chongqing Medical University,Chongqing 401331,China

出　　处：《Journal of Integrative Medicine》2021年第1期56-65,共10页结合医学学报（英文版）

基　　金：supported by Wenzhou Science and Technology Bureau Project(to Zhu Liqing,No.Y20190088)。

摘　　要：Objective:High-fat diet is one of the main risk factors that disrupt the balance of gut microbiota,which eventually will induce colorectal cancer(CRC).Evodiamine(EVO)is a wildly used multifunctional traditional Chinese medicine extract.In this study,we investigated the role of gut microbiota in high-fat dietpropelled CRC and the potential of EVO for CRC chemoprevention.Methods:Gut microbiota,serum D-lactic acid and endotoxin from 38 patients with colon cancer and 18 healthy subjects were detected by quantitative real-time polymerase chain reaction and enzyme-linked immunosorbent assay(ELISA).In addition,body mass index,phospho-signal transducer and activator of transcription 3(p-STAT3)expression in cancer tissues and paracancerous tissues were detected by immunohistochemistry.A mouse intestinal inflammatory tumor model was established by azomethane/sodium dextran sulfate,followed by treatment with EVO and 5-aminosalicylic acid(ASA).Gut microbiota and inflammatory factors were detected by quantitative polymerase chain reaction,while serum D-lactic acid and endotoxin were detected by ELISA.Furthermore,cell proliferation,cell apoptosis,and interleukin(IL)-6/STAT3/P65 pathway were evaluated by 5-ethynyl-20-deoxyuridine,terminal-deoxynucleotidyl transferase-mediated nick-end labeling,and Western blot assays.Results:In patients with colon cancer,the numbers of Enterococcus faecalis and Escherichia coli were increased,while those of Bifidobacterium,Campylobacter and Lactobacillus were decreased.Serum endotoxin and D-lactic acid levels and p-STAT3 levels were significantly increased.In the mouse model,both EVO and ASA inhibited tumor formation,decreased the proliferation of tumor cells,and induced apoptosis of tumor cells.Compared with the control group,the numbers of E.faecalis and E.coli were decreased,while Bifidobacterium,Campylobacter and Lactobacillus numbers were increased.In the EVO group,serum endotoxin and D-lactic acid levels and inflammatory factors were significantly decreased.Further,the IL6/STAT3/P65 signa

关 键 词：EVODIAMINE Gut microbiota Colitis-associated cancer Signal transducer and activator of transcription 3 

分 类 号：R285[医药卫生—中药学]