微生物法测定婴幼儿乳粉中维生素B12含量的不确定度评定  

Uncertainty evaluation for determination of vitamin B in infant milk powder by microbiological method

作　　者：罗彤 付文雯 黄坤 朱影 王鸣秋 刘艳 李诗瑶 邵翠翠 朱必婷 何名扬 马弋 LUO Tong;FU Wen-Wen;HUANG Kun;ZHU Ying;WANG Ming-Qiu;LIU Yan;LI Shi-Yao;SHAO Cui-Cui;ZHU Bi-Ting;HE Ming-Yang;MA Yi(Hubei Provincial Institute for Food Supervision and Test,Wuhan 430075,China;Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test,Wuhan 430075,China)

机构地区：[1]湖北省食品质量安全监督检验研究院,武汉430075 [2]湖北省食品质量安全检测工程技术研究中心,武汉430075

出　　处：《食品安全质量检测学报》2021年第1期94-100,共7页Journal of Food Safety and Quality

摘　　要：目的建立微生物法测定婴幼儿乳粉中维生素B_(12)含量的不确定评定方法。方法依据JJF1059.1—2012《测量不确定度评定与表示》及GB 5413.14—2010《食品安全国家标准婴幼儿食品和乳品中维生素B_(12)的测定》分析微生物法测定婴幼儿乳粉中维生素B_(12)含量过程中产生的各测量不确定度分量,计算测量结果的合成标准不确定度和扩展不确定度。结果当样品中维生素B_(12)含量为3.97μg/100 g时,其扩展不确定度为0.53μg/100 g(k=2)。结论标准溶液配制和标准曲线拟合计算待测溶液含量产生的不确定度对检测结果影响较大。Objective To establish an uncertain assessment method for the determination of vitamin B_(12)in infant milk powder by microbiological method.Methods According to JJF 1059.1—2012 Evaluation and representation of measurement uncertainty and GB 5413.14—2010 National food safety standard for the determination of vitamin B_(12)in infant food and dairy products,the components of measurement uncertainty generated during the determination of vitamin B_(12)in infant milk powder by microbial method were analyzed,and the synthetic standard uncertainty and extended uncertainty of the measured results were calculated.Results When the vitamin B_(12)content of the sample was 3.97 g/100 g,the expansion uncertainty was 0.53 g/100 g(k=2).Conclusion Theuncertaintiescaused by the preparation of standard solution and the calculation of standard curve fitting havea great influence on the test results.

关 键 词：微生物法 婴幼儿乳粉 维生素B_(12) 不确定度 莱士曼氏乳酸杆菌 

分 类 号：TS252.7[轻工技术与工程—农产品加工及贮藏工程]