湘莲莲房中主要生物碱成分提取方法优化与含量测定  

Optimization of extraction methods and determination of main alkaloids in seedpod of Hunan lotus

作　　者：雷德卿 胡红玲[2] 王子倩 LEI De-Qing;HU Hong-Ling;WANG Zi-Qian(Changsha Institute for Food and Drug Control,Changsha 410075,China;Xiangya Hospital,Central South University,Changsha 410008,China;Changsha Medical College,Changsha 410089,China)

机构地区：[1]长沙市食品药品检验所,长沙410075 [2]中南大学湘雅医院,长沙410008 [3]长沙医学院,长沙410089

出　　处：《食品安全质量检测学报》2021年第1期332-337,共6页Journal of Food Safety and Quality

摘　　要：目的以莲心碱、异莲心碱、甲基莲心碱为湘莲莲房质量评价指标,优化提取工艺参数,建立高效液相色谱法同时测定3种物质。方法用酸性80%乙醇作溶剂超声提取莲房生物碱类成分,碱化后分离定量。经Waters XSelect C_(18)色谱柱(250 mm×4.6 mm,5μm)进行分离,以甲醇-0.05 mol/L磷酸二氢钠为流动相进行梯度洗脱。结果莲心碱、异莲心碱、甲基莲心碱分别在1.994~79.76μg/mL(r^(2)=0.9993,n=6)、1.951~78.05μg/mL(r^(2)=0.9996,n=6)、1.972~78.87μg/mL(r^(2)=0.9998,n=6)范围内线性关系良好,回收率分别为99.5%、98.2%、98.7%(n=9)。结论该方法简便、回收率高,实验结果为莲房中生物碱定量分析和湘莲莲房质量评价提供参考。Objective To optimize the extraction process and establish high performance liquid chromatography method for simultaneous determination of liensinine,isoliensinine and neferine in seedpod of Hunan lotus.Methods The alkaloids were extracted by ultrasonic with acid 80%ethanol,and extracting solution was separated after alkalization.Waters XSelect C_(18)column(250 mm×4.6 mm,5μm)was used for separation,gradient elution was performed with methanol-0.05 mol/L sodium dihydrogen phosphate as mobile phase.Results The liensinine,isoliensinine and neferine showed good linear ranges at 1.994-79.76μg/mL(r^(2)=0.9993,n=6),1.951-78.05μg/mL(r^(2)=0.9996,n=6),1.972-78.87μg/mL(r^(2)=0.9998,n=6),and the average recoveries were 99.5%,98.2%,98.7%(n=9).Conclusion This method is simple and has excellent recovery,the experimental results provide reference for the quantitative analysis of alkaloids in the Hunan lotus and the quality evaluation of the hunan lotus.

关 键 词：高效液相色谱法 莲心碱 异莲心碱 甲基莲心碱 湘莲莲房 

分 类 号：O657.72[理学—分析化学] R284[理学—化学]