去势法联合慢性不可预知性温和应激法建立围绝经期综合征肝郁证动物模型的研究  

Castration combined with chronic unpredictable mild stress to establish animal model of liver depression pattern of perimenopausal syndrome

作　　者：阮璐薇 张鹏横 黄睿婷 沈建英[1,2,3] 梁文娜 RUAN Lu-wei;ZHANG Peng-heng;HUANG Rui-ting;SHEN Jian-ying;LIANG Wen-na(Chinese Medicine School,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;Fiuijian Province Collaborative Innovation Center of TCM Health Management in 2011,Fuzhou 350122,,Chinax;Key Laboratory of TCM Health Status Identification in Fujian Province,Fuzhou 350122,China)

机构地区：[1]福建中医药大学中医学院,福州350122 [2]福建省2011中医健康管理协同创新中心,福州350122 [3]福建省中医健康状态辨识重点实验室,福州350122

出　　处：《中华中医药杂志》2021年第1期394-397,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81774209,No.81673882);福建省科技厅社会发展引导性项目(No.2019Y0039)。

摘　　要：目的:采用去势法联合慢性不可预知性温和应激法(CUMS)建立围绝经期综合征肝郁证大鼠动物模型。方法:SPF级雌性3月龄SD大鼠30只,按照体质量分层随机分为假手术组、去势组以及模型组,每组10只。假手术组大鼠摘除卵巢旁脂肪组织,去势组及模型组大鼠行去势手术,采用苏木素-伊红染色(HE)观察大鼠阴道脱落细胞涂片,判断是否成功建立围绝经期综合征动物模型。模型组大鼠进行CUMS建立肝郁证模型。记录大鼠一般情况,观察体质量、强迫游泳不动时间及糖水偏好率的变化。结果:模型组大鼠精神倦怠、反应迟缓、毛色暗淡粗糙、进食量少。阴道脱落细胞涂片提示去势后大鼠失去规律动情周期变化;大鼠体质量、体质量增长率,去势组均显著高于假手术组(P<0.05,P<0.01),而模型组则显著低于去势组(P<0.01)。行为学表现方面,与假手术组比较,模型组大鼠的强迫游泳不动时间延长(P<0.05)、糖水偏好度显著降低(P<0.01)。结论:采用去势法联合CUMS可建立围绝经期综合征肝郁证动物模型,其行为特征与肝郁证的中医病理特点相似。Objective:To establish an animal model of liver depression pattern of perimenopausal syndrome by using castration operation combined with chronic unpredictable mild stress(CUMS).Methods:Thirty 3-month-old SPF female SD rats were randomly divided into sham group(n=10),castration group(n=10)and model group(n=10)according to body weight.Rats in the sham group were removed of para-ovarian adipose tissue,castrated group and model group rats were castrated,and hematoxylin-eosin staining(HE)was used to observe the smear of vaginal exfoliated cells of the rats to determine whether they were successful to establish animal models of perimenopausal syndrome.Rats in the model group underwent CUMS to establish a liver-stagnation pattern model.Record the general condition of rats and observe the changes of body weight,forced swimming immobility time and sugar water preference rate.Results:The rats in the model group had mental fatigue,slow response,dull and rough coat,and low food intake.Vaginal exfoliated cell smears show that rats lose regular rhythm cycle after ovariectomy;the weight and growth rate of rats in castration group were higher than that in sham operation group(P<0.05,P<0.01),while model group was significantly lower than castration group(P<0.01).Compared with the sham operation group,the model group’s forced swimming immobility time was prolonged(P<0.05)and the sugar water preference was significantly reduced(P<0.01).Conclusion:Castration combined with CUMS can establish an animal model of perimenopausal syndrome with liver depression syndrome,and its behavioral characteristics are similar to those of traditional Chinese medicine pathology of liver depression syndrome.

关 键 词：围绝经期综合征 肝郁证 慢性不可预知性温和应激 动物模型 去势法 

分 类 号：R-332[医药卫生] R271.116