蚕豆蛋白酶解物分离纯化及降胆固醇活性  

Separation and purification of broad bean protein hydrolysate and its cholesterol lowering activity

作　　者：陈丹阳 韩涛[1] CHEN Danyang;HAN Tao(College of Food Science and Engineering,Beijing University of Agriculture,Beijing 102206,China)

机构地区：[1]北京农学院食品科学与工程学院,北京102206

出　　处：《中国油脂》2021年第1期61-64,104,共5页China Oils and Fats

摘　　要：采用凝胶过滤色谱对经过大孔吸附树脂纯化的蚕豆蛋白酶解物进行分离纯化,以期得到高降胆固醇活性的酶解物组分。结果表明:单因素试验得到蚕豆蛋白酶解物凝胶过滤色谱最佳分离纯化工艺条件为Sep G-10、G-25葡聚糖凝胶为柱填充材料,10 mg/m L的酶解液上样量4 m L,洗脱剂为去离子水,洗脱流速1 m L/min。凝胶过滤色谱分离纯化后得到F1~F6 6个蚕豆蛋白酶解物组分;与10 mg/m L考来烯胺散阳性对照比较,F3、F6组分对3种胆酸盐(胆酸钠、甘氨胆钠酸、牛磺胆酸钠)抑制率均高于阳性对照,其中F6组分的相对抑制率最高,分别为(274.98±0.19)%、(140.22±0.20)%、(130.99±0.22)%。The broad bean protein hydrolysate purified by macroporous adsorption resin was separated and purified by gel filtration chromatography to obtain broad bean protein hydrolysate with cholesterol lowering activity. The results showed that the optimal separation and purification conditions of the broad bean protein hydrolysate by single factor experiment were obtained as follows: with Sep G-10 and G-25 dextran gel as the column filling material,loading amount of 10 mg/m L enzymatic hydrolysate 4 m L,deionized water as mobile phase,elution speed 1 m L/min. After separated and purified by the gel filtration chromatography,the components of F1-F6 six broad bean protein hydrolysates were obtained.Compared with the 10 mg/m L of coletenamine powder positive control,the inhibitory rates of F3 and F6 components to three kinds of cholate salts( sodium cholate,soldium glycocholate,sodium taurocholate)were higher than those of the positive control. The relative inhibition rate of F6 component was the highest,which was( 274. 98 ± 0. 19) %,( 140. 22 ± 0. 20) % and( 130. 99 ± 0. 22) % respectively.

关 键 词：蚕豆 蚕豆蛋白酶解物 胆固醇 凝胶过滤色谱 分离纯化 

分 类 号：TS214[轻工技术与工程—粮食、油脂及植物蛋白工程] TS218[轻工技术与工程—食品科学与工程]