软枣猕猴桃不定根总黄酮提取工艺的优化  

Optimization of extraction technology of total flavonoids from adventitious roots of Actinidia arguta

作　　者：吴晓晗 李学峰 范明智 廉美兰[1] 金美玉[1] WU Xiaohan;LI Xuefeng;FAN Mingzhi;LI Meilan;JIN Meiyu(Agricultural College of Yanbian University,Yanji Jilin 133002,China)

机构地区：[1]延边大学农学院,吉林延吉133002

出　　处：《延边大学农学学报》2021年第1期18-23,共6页Agricultural Science Journal of Yanbian University

基　　金：国家自然科学基金(81960685);吉林省科技发展计划项目(20180101278JC)。

摘　　要：为优化软枣猕猴桃不定根中总黄酮的最佳提取工艺,该试验以生物反应器培养的软枣猕猴桃不定根为试验材料,将不同的提取溶剂、提取浓度、提取时间、提取温度、提取次数和料液比条件下的提取总黄酮量作为评价指标,采用正交试验设计对总黄酮的提取工艺进行优化。结果表明:利用75%的乙醇进行提取,在提取时间1 h、提取温度50℃、提取次数3次、料液比1∶30(g∶mL)时,提取的总黄酮量最高,达到149.97 mg/g。In order to optimize the extraction process of total flavonoids from the adventitious roots of Actinidiaarguta,the adventitious roots of Actinidiachinensis cultured in bioreactor as materials,the content of total flavonoids was taken as the evaluation index,which extracted in different extraction solvents,extraction concentration,extraction time,extraction temperature,extraction times and ratio.The results showed the content of total flavonoids reached 149.97 mg·g-1,when the extraction time was 50℃,the extraction temperature was 50 times,the extraction time was 3 times,and the ratio of material to liquid was 1∶30(g∶mL).

关 键 词：软枣猕猴桃 不定根 总黄酮 提取工艺 

分 类 号：S663.4[农业科学—果树学]