消毒剂胁迫鼠伤寒沙门氏菌ATCC 14028s的抗性及耐药性测定  

The Resistance and Drug Resistance of Salmonella Typhimurium ATCC 14028s by Disinfectants Stress

作　　者：吴上 杨阳 孙嘉笛 杨嘉 张银志[1] 孙秀兰[1] 纪剑 WU Shang;YANG Yang;SUN Jia-di;YANG Jia;ZHANG Yin-zhi;SUN Xiu-lan;JI Jian(School of Food Science and Technology of Jiangnan University,Wuxi 214122,China;Yangzhou Center for Food and Drug Control,Yangzhou 225000,China)

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]扬州市食品药品检验检测中心,江苏扬州225000

出　　处：《山东农业大学学报（自然科学版）》2021年第1期13-18,共6页Journal of Shandong Agricultural University：Natural Science Edition

基　　金：“十三五”国家重点研发计划(2017YFC1600102);2020年度江苏省市场监督管理局科技计划项目(KJ204139)。

摘　　要：为探索消毒剂胁迫后的鼠伤寒沙门氏菌ATCC14028s的抗性及对抗生素的耐药性,本研究选取次氯酸钠、氯已定(97%)、苯扎氯铵(80%)和十六烷基氯化吡啶(≥98%)分别胁迫鼠伤寒沙门氏菌ATCC14028s,胁迫后菌株经消毒剂抗性测定及抗生素耐药性测定。结果表明,胁迫后的沙门氏菌,对4种消毒剂的抗性几乎不变,而对部分抗生素的抗性产生显著变化,其中经氯已定、次氯酸钠胁迫的菌株对环丙沙星和氨苄西林的耐药性显著增强,对庆大霉素的耐药性减弱;苯扎氯铵、十六烷基氯化吡啶胁迫的菌株对环丙沙星、四环素和庆大霉素的耐药性提升。因此,消毒剂的过度使用,对食品加工中耐药菌的防控具有理论意义。To explore the disinfectant after the stress of Salmonella Typhimurium ATCC14028s resistance and resistance to antibiotics,this study selected Sodium Hypochlorite,Chlorhexidine(97%),Benzalkonium Chloride(80%)and Cetyl Pyridine Chloride(98%or higher)respectively stress of Salmonella Typhimurium ATCC14028s,stress strain after the determination of disinfectant resistance and antibiotic resistance.The results showed that the resistance of Salmonella to four kinds of disinfectants was almost unchanged after stress,but the resistance to some antibiotics changed significantly.The resistance of Salmonella strains to Ciprofloxacin and Ampicillin increased significantly after stress of Chlorhexidine and Sodium Hypochlorite,and the resistance to Gentamicin decreased.The resistance to Ciprofloxacin,Tetracycline and Gentamicin of strains stressed by Benzalkonium Chloride and Cetylpyridine Chloride was increased.Therefore,the overuse of disinfectants has theoretical significance for the prevention and control of drug-resistant bacteria in food processing.

关 键 词：沙门氏菌 消毒剂 抗性 耐药性 

分 类 号：R378.22[医药卫生—病原生物学]