激光照射频次和单次照射时长对眼部组织的损伤作用评估  

Evaluation of eye injury degree of laser irradiation frequency and single duration

作　　者：张宇飞 危冬昱 王伟 刘大铭 任泽 李向前 陈涛 张作明 Zhang Yufei;Wei Dongyu;Wang Wei;Liu Daming;Ren Ze;Li Xiangqian;Chen Tao;Zhang Zuoming(Center of Clinical Aerospace Medicine,Air Force Military Medical University,Xi'an 710032,China;Naval Medical Center of PLA,Shanghai 200433,China;Basic Medical College,Air Force Military Medical University,Xi'an 710032,China)

机构地区：[1]空军军医大学航空航天临床医学中心,航空航天医学教育部重点实验室,西安710032 [2]海军军医大学海军特色医学中心,上海200433 [3]空军军医大学基础医学院,西安710032

出　　处：《中华实验眼科杂志》2021年第3期184-190,共7页Chinese Journal Of Experimental Ophthalmology

基　　金：装备科研重点项目(172B02027);陕西省重点研发项目(2018SF-257)。

摘　　要：目的探讨激光照射频次和单次照射时长对泪液分泌、晶状体以及视网膜形态和功能的影响。方法选取36只健康豚鼠进行眼部激光照射实验,采用随机数字表法并依据激光照射频次和单次照射持续时间的不同将豚鼠随机分为高频短时(HFST)组、高频长时(HFLT)组、中频短时(MFST)组、中频长时(HFLT)组、低频短时(LFST)组和低频长时(LFLT)组,每组6只。各组豚鼠右眼进行500 lx的激光照射作为实验眼,左眼不接受任何干预作为对照眼。高频激光照射为15次,中频照射为10次,低频照射为5次,每次照射时间间隔为10 min;短时照射为30 s/次,长时照射为60 s/次。采用基础泪液分泌试验(SⅠt)对各组豚鼠实验眼与对照眼间泪液分泌量进行测定和比较;采用裂隙灯显微镜斜照法评估各组豚鼠晶状体透明性变化情况;采用眼底照相法评估豚鼠眼底和视盘大致形态;采用视网膜电图(ERG)记录法评价各组豚鼠视网膜功能变化;采用常规组织病理学方法检查视网膜外核层厚度变化。结果HFST组、HFLT组、MFST组、MFLT组、LFST组和LFLT组实验眼泪液分泌量分别为8.00(7.37,9.00)、8.75(8.25,9.00)、8.50(7.75,9.50)、9.00(8.50,9.50)、8.00(7.37,8.75)和8.25(7.75,8.75)mm/5 min,总体比较差异无统计学意义(χ^(2)=5.502,P=0.240);各组豚鼠实验眼泪液分泌量与对照眼比较差异均无统计学意义(均P>0.05)。各组豚鼠双眼晶状体均透明,实验前后眼底均未见出血和渗出;HFST组实验眼ERG暗适应3.0 a波振幅值低于LFST组,差异有统计学意义(P<0.05),各组间实验眼ERG暗适应3.0 b波振幅总体比较差异无统计学意义(F=1.358,P=0.268);各组豚鼠实验眼与对照眼间ERG a、b波振幅比较差异均无统计学意义(均P>0.05);各组间豚鼠实验眼视网膜外核层厚度总体比较差异无统计学意义(F=0.952,P=0.463)。结论500 lx激光照射对眼表组织和晶状体无明显损伤,但一定程度上造成视网膜功能�Objective To explore the effects of laser irradiation parameters(irradiation frequency and single duration)on tear secretion,lens and retina.Methods Thirty-six healthy guinea pigs were randomly divided into 6 groups with random number table method according to different frequency and single exposure duration of laser to the eye,namely,high frequency short time(HFST)group,high frequency long time(HFLT)group,medium frequency short time(MFST)group,medium frequency long time(MFLT)group,low frequency short time(LFST)group and low frequency long time(LFLT)group,6 for each group.The right eyes were irradiated with 500 lx laser as experimental eyes,and the left eyes of the guinea pigs served as the control eyes.The high,medium and low irradiation frequencies were defined as 15 times,10 times and 5 times,respectively,and the short and long period was defined as 30 seconds and 60 seconds each time,respectively.The right eyes were irradiated based on the grouping at a 10-minute interval.The tear secretion was detected by SchirmerⅠtest;lens opacity was assessed under the slit-lamp microscope;fundus photography was performed to evaluate the general morphology of retina;retinal function was evaluated by electroretinogram(ERG)record and the thickness of retinal outer nuclear layer was measured by histopathology examination.This study protocol was approved by the Medical Ethics Committee of Air Force Military Medical University(No.20181203),and the use and care of the experimental animals complied with the ARVO statement.Results The tear secretion was 8.00(7.37,9.00),8.75(8.25,9.00),8.50(7.75,9.50),9.00(8.50,9.50),8.00(7.37,8.75)and 8.25(7.75,8.75)mm/5 min in the HFST group,HFLT group,MFST group,MFLT group,LFST group and LFLT group,respectively,without significant difference among the groups(χ^(2)=5.502,P=0.240);after laser irradiation,there were no statistically significant differences in tear secretion between the control eyes and laser-irradiated eyes in all the groups(all at P>0.05).The lenses were clear and the fundus wa

关 键 词：激光 眼部损伤 视网膜电图 视网膜功能 

分 类 号：R779.12[医药卫生—眼科]