姜烯酚6衍生物M14通过调控Keap1/Nrf2信号通路诱导人结直肠癌HCT-8细胞凋亡  

Gingerol 6 Derivative M14 Induces Human Colorectal Cancer HCT-8 Cells Apoptosis through Keap1/Nrf2 Signaling Pathway

作　　者：杨春[1] 李恒[1] 董欣宇 谢小亮[1] 张东[1] YANG Chun;LI Heng;DONG Xinyu;XIE Xiaoliang;ZHANG Dong(Department of Colorectal Surgery,the General Hospital of Ningxia Medical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学总医院结直肠外科,银川750004

出　　处：《宁夏医科大学学报》2021年第2期109-112,128,共5页Journal of Ningxia Medical University

基　　金：宁夏医科大学优势学科群项目(XY201509;XY201507)。

摘　　要：目的探讨姜烯酚6衍生物M14(S6-M14)诱导人结直肠癌HCT-8细胞凋亡的作用及可能的分子机制。方法MTT法检测不同浓度的姜烯酚6衍生物M14对人结直肠癌HCT-8细胞增殖的影响;ATP显色法检测姜烯酚6衍生物M14促进人结直肠癌HCT-8细胞凋亡的剂量依赖效应;Western blot检测胞质内Keap1和Nrf2蛋白及核内Nrf2蛋白的表达。结果0.25、0.5、1、2、4 mmol·L^(-1)S6-M14均可抑制人结直肠癌HCT-8细胞的增殖(P均<0.001);姜烯酚6衍生物M14剂量依赖性地诱导人结直肠癌HCT-8细胞凋亡;姜烯酚6衍生物M14下调人结直肠癌HCT-8细胞胞质内Keap1和Nrf2蛋白表达,促进Nrf2蛋白的核易位(P均<0.05)。结论姜烯酚6衍生物M14抑制人结直肠癌HCT-8细胞增殖、诱导细胞凋亡,调控Keap1/Nrf2信号通路,有潜在的预防结直肠癌发生的作用。Objective To study the effect of Gingerol 6 derivative M14(S6-M14)on human colorectal cancer HCT-8 cell apoptosis and its possible molecular mechanism.Methods MTT assay was used to determine the effect of different concentrations of S6-M14 on the activity of human colorectal cancer HCT-8 cells.The dose-dependent effect of S6-M14 on the apoptosis of human colorectal cancer HCT-8 cells was detected by The ATP chromogenic method.Keap1/Nrf2 signaling pathway protein expression was analyzed by Western blot.Results 0.25,0.5,1,2 and 4 mmol·L^(-1) S6-M14 all inhibited the activity of human colorectal cancer HCT-8 cell(P all<0.001).S6-M14 dose-dependently inducted apoptosis of human colorectal cancer HCT-8 cell.S6-M14 down-regulates the expression of Keap1 and Nrf2 proteins in the cytoplasm of human colorectal cancer HCT-8 cells,and promotes Nrf2 protein nuclear translocation(P all<0.05).Conclusion S6-M14 can prevent colon cancer by inhibiting human colorectal cancer HCT-8 cell proliferation,inducing apoptosis,and regulating Keap1/Nrf2 signaling pathway.

关 键 词：姜烯酚6衍生物M14 人结直肠癌HCT-8细胞 细胞凋亡 Keap1/Nrf2信号通路 

分 类 号：R735.35[医药卫生—肿瘤]